EXPERIMENT STATION BULLETINS. 333 



Date. Weight. Date. Weight. 



Feb. 2, 181)7 885 Jvily l(i, 1897 048 



March 2, 1897 837 July 17, 1897 627 



April 2, 1897 836 Julv 18, 1897 630 



May 2, 1897 818 JulV 19, 1897 623 



June 2, 1897 803 July 24, 1897 600 



July 2. 1897 755 



She was not weighed after this. 



Her death occurred July 29, 18!)7. Her lungs were a solid mass of tubercles. 

 Tubercles of various sizes occurred throughout the abdominal cavity, some were three 

 inches in diameter. The lymphatic glands and the pleu»al and abdominal cavities 

 were generally att'ected. The udder had begun to show signs of tuberculosis, but in the 

 incipient stage. 



AIDA 2, 0112. 



She was the daughter of Orient, 2567, and Aida. 3318. and was born January 12. 

 1892. She was a (iuernsev. She lirst reacted April 21, 1897. Her test records are: 



April 20, 1897, before "injection. 102.2, 101.7, 100.4, 100.1, 104.0. 



.\pril 21, 1897, after injection, 101.2, 100.7, 100.8, 101.4, 102.6, 105.4, lOG.O, 103.7, 

 106.0, 104.4. 



October 13, 1897, before injection, 101.6, 99.9, lOl.G, 102.5, 102.3, 102.0, 101.6. 



October 14, 1897. after injection, 104.2, 104.0, 103.7, 105.2, 105.3, 105.0, 105.4, 104.9, 

 104.6, 105.0, 104.4. 



March 31. 1898, before injection, 100.0, 98.6, 98.1, 97.0, 98.6, 96.9, 98.6, 96.9, 96.9, 

 100.6. 



April 1, 1898, after injection, 100.6, 100.9, 102.3, 101.4, 101.4, 101.6, 103.5, 101.9, 101.4. 

 99.1, 99.0, 99.0. 



October 14, 1898, before injection, 100.2, 98.0, 98.3, 99.2, 100.8, 99.9, 98.5. 99.4, 99.6, 

 101.6. 



October 15, 1898, after injection, 99.5, 99.6, 100.6, 103.1, 103.1, 102.6, 104.0. 103.2. 

 103.0, 102.6. 



March 30, 1899, before injection, 99.8, 99.0, 97.8, 99.1, 98.4, 98.6, 96.1. 98.4, 99.6, 98.5. 



March 31, 1899, after injection, 97.2, 99.1, 98.8, 98.4, 101.7, 9.5.6, 102.2. 102.9, 103.7, 

 103.0, 103.6, 100.7. 



Of the several tests made for tubercle bacilli in her milk. 1 have secured only one 

 positive result. This positive result, I am inclined to think, was probably due to 

 the contraction of the disease by the guinea pig from other diseased guinea pigs. 

 Although this seldom happens, in this case the evidence seems to favor such a con- 

 clusion, for the milk came from the right front quarter, and when tested several times 

 after, it never set up tuberculosis. This was shown in another way. A litter of four 

 pigs was taken, four weeks old. and separated into two lots of two each. To one 

 lot was fed Aida's milk and some meal. To the other lot no milk was fed. The duration 

 of this test was six months, at the end of which time all the pigs were killed, but none 

 of them possessed any tubercles. This would help substantiate the above conclusion. 

 In all probability, the one positive test was due to some outside contamination and 

 her milk was free from tubercle bacilli. 



Examinations of her feces, both microscopical and inoculative, always resulted nega- 

 tively. 



Her urine was always free from the virus of this disease, as demonstrated by re- 

 peated inoculations. 



Her calves were College Cross, born November 2, 1896, and College Cross, born 

 October 22, 1897, both of which were tested April 21, 1897, and April 1, 1898, 

 respectively, but neither reacted. 



On April 18, 1899, Aida 2d was killed. At the time of killing she was in a very fair 

 condition. The post-mortem examination revealed several hard, cheesy tubercles in 

 the apex of one of her lungs and the adjacent tissue highly inflamed. The mediastinal 

 glands had deposits of tiiberculous calcareous material. A tuberculous mesenteric gland 

 was also found. 



