PEACH YELLOWS AND PEACH ROSETTE. 171 



into each tree. Its slow progress through the tissues is inferred from the 

 fact that no symptoms were visible until after three months, and probably 

 none until the following May or June — eight to nine months after the 

 date of inoculation. It should also be noted that the inserted buds pro- 

 duced the same effect, although cut from many different trees. 



Experiment 2. — The trees selected for this series of inoculations were 

 much like those used in No. 1. They were grown from Tennessee seed 

 and formed part of a large nursery owned by Thomas J. Shallcross, 

 Locust Grove, Maryland. The seedlings numbered about 100,000, and 

 were very thrifty. I first saw them at budding time, when they were 

 about five months old. The trees devoted to this experiment were two 

 outer rows, not different in appearance from the rest. 



The buds for inoculation were cut from an orchard on the same farm. 

 They came from healthy-looking shoots on a vigorous six-year-old tree. 

 This tree had shown no symptoms of disease until that summer, when 

 some of its limbs bore premature fruit and the characteristic shoots. One 

 limb of the tree was badly diseased only a short distance from where the 

 shoots were cut. 



The buds were cut August 3, 1887, and inserted the same day. One bud 

 only was put into each tree. The manner of inoculation was substantially 

 like that already described. Subsequent examination showed that a union 

 between bud and stock had taken place in every instance, or nearly every 

 one, the budder having been very expert. No buds pushed and no symp- 

 toms of disease developed that autumn. 



In the spring of 1888, while the buds were yet dormant, these trees were 

 removed to Hubbardston, Michigan, and set ten by ten feet apart, princip- 

 ally upon a moderately fertile, sandy loam, which had been used for a 

 garden. This location was selected as, on the whole, the best that could 

 be had at that time. The soil has been cleared of forest and brought under 

 cultivation since 1850. There were and are no known cases of yellows 

 within forty miles, and the nearest commercial orchards are twenty miles 

 away, and not extensive. By long residence I am familiar with the whole 

 country, and believe that the only peach trees in that township or the 

 adjoining ones are such as have been planted very sparingly in gardens or 

 around houses. To most farms and gardens this tree is an entire stranger. 

 The principal objection to the location, and a serious one, was the danger 

 from low winter temperatures and sudden fluctuations, which in the past 

 had frequently killed peach trees to the ground, and consequently had 

 long since very effectually discouraged planting. However, the risk was 

 taken, and, as good fortune would have it, the winters of 1889 and 1890 

 were so mild that the trees did not suffer in the least. 



Altogether, three hundred and thirty-six trees were set, and upon my 

 father's place, that I might have the land more fully under control. Two 

 hundred and ten were the inoculated trees already mentioned and one 

 hundred and twenty-six were unbudded seedlings from the same nursery 

 to be used in making comparisons. 



These trees were set out April 24, under personal direction, and were 

 examined carefully, one by one, as late as June 26 of that year. At that 

 date there was no difference in the appearance of the two lots. Six of 

 each were dead, apparently from the shock of transplanting. The remainder 

 of the unbudded trees were perfectly healthy; and the remainder of 

 the inoculated ones appeared to be so, with the exception of one tree 

 which began to look suspicious. In sixty per cent of the inoculated trees 



