SEED STERILIZATION AND ITS EFFECT UPON INOCULATION. 



Table I. — Gcnnination of .^ccds (tfter trcafiiictit vUh vdrioits <]isinfcTta)it.'i — 



CoutiiuK'd. 



Table II. — Effect of (li.sinfectant.'^ in rendering .seeds free from bacterial in- 

 fection, the minus sign indicating the absence of infection. 



Seed. 



Alfalfa 



Crimson clover . 



Garden pea 



Bean 



Wheat 



Corn 



Radish 



Hydrogen peroxid, 

 1 per cent." 



10 

 minutes. 



60 

 minutes. 



Formaldehyde, 10 per cent. 6 



Check. 



+ 

 + 

 + 

 + 

 + 

 + 



•2 I 15 

 minutes, minutes. 



+ 



Seed. 



Alfalfa 



Crimson clover. 



Garden pea 



Bean 



Wheat 



Corn 



Radish 



Formaldehyde, 1 per 

 cent. 



minutes. 



15 

 minutes. 



Mercuric chlorid, 0.5 per cent. 



Check. 



+ 

 + 

 + 

 + 

 + 

 + 



minutes. 



30 



minutes. 



Mercuric chlorid, 0.1 

 per cent. 



5 30 



minutes. minutes. 



+ 



+ 

 + 



+ 



+ 



" 1 per cent hydrogen peroxid ^1 part commercial H2O2 (3 per cent) in 2 parts of water. 

 '' 10 per cent formaldehyde =1 part of formalin (40 per cent) in 3 parts of water. 



The figures concerning germination when taken alone do not indi- 

 cate the actual differences shown by the various series. Many of the 

 seeds treated with formaldehyde and mercuric chlorid produced dis- 

 torted sprouts, showing various degrees of injury, while in the per- 

 oxid series the sprouts were usually larger and better in api^earance 

 than those in the untreated series, and the seed germinated more 

 quickl}^ 



[Cir. 07] 



