Bums and Hedden, Conditious iiiflueucingregeneration of hypocotyl. 080 



Adventitious buds were also produced on tlie epicotyl when 

 the main Vegetation point was cut away. In this case the buds 

 in the axil of the cotyledons develop, but their development 

 appears to have no influence on the development op „adventi- 

 tious" buds. 



Material and methods. This paper deals largely with 

 conditions intiuencing the production of adventitious buds on 

 the hypocotyl. 



The i)lants used were L'iuana hipartita .splendida^ Antirrhinum 

 majus and Linum iisitafisslmitm. 



The cotyledons were cut away just below their insertion; 

 usually when the seedling was from two to three cm high. 



After this Operation the plants were placed under various 

 external conditions which will be taken up separately. 



Oß 



c 



Fig. 2. 

 Lii/aria a b. The fotyledons were removed from a;l) was not injured. 

 Note dif ference in number of leaves in regenerated sliort. Aniirrhinum c and d. 

 The Order of shoofe is shown bj^the iigures. Note size, number and arrange- 



ment of leaves in regenerated buds. 



The experiments Avere started in November 1903 and carried 

 on through the following year. 



The results obtained contirm those of Küster. All indi- 

 viduals of the plant named whose cotyledons and part of the 

 hypocotyl were removed produced numerous buds. 



General description. In the course of from 5—9 days 

 after the Operation the hypocotyl buds began to appear as 

 small ernption on the epidormis. A day or two before the buds 

 a])pear, the hypocotyl becomcs somewhat swoUcn. 



The number of these buds varied in the three different 

 plants L'inaria produced from 1 — 6 buds, Auf irr// in um from 1 — 14, 

 and Linuiii from 1 — HO or more. Ever\^ epidermal cell of L?«r«>« 



