218 STATE BOARD OF AGRICULTURE. 



112— G. 

 115—1. 

 110— J. 

 117— L. 



No. 3. 



Source. — From Pasteurized milk not recorded in this bulletin. Method of Pasteur- 

 izing, same as in first half of samples. 



Form and grouping. — Bacillus. Its thickness varies from f m to 1 m: its length 

 from 2| m to 6 m. Ends are round. It may form in short threads, but is usually in 

 pairs or single. 



S/inres. — It forms median spores. 



Protoplasm. — The protoplasm of this bacillus is usually homogeneous, but just 

 before sporulation it becomes markedly granular. 



T( mperature. — Grows best at 28° C. 15° C. checks its growth materially, scarcely 

 developing at all. Withstands 93° C. for 20 min., but is killed at 9(5° C. for the same 

 time. 



Motion. — Very motile. It has a progressive tumbling motion. 



Colonies. — The border is irregular and broken, yet its outline is sharp and distirict. 

 Instead of spreading out in a thin plaque, it is rather abrupt. From the border are 

 well-defined lines much darker than the surrounding mass, which run to the center. 

 These markings resemble small crevices extending from the border to the center. 

 At the center there is a dark mass which obliterates all the markings. The whole 

 effect is much like looking down upon a summer rose with its stamens and conylom- 

 erate center from which the petals radiate. The color of the colony is dark yellowish 

 bi'own . 



Gelatin tube-cultures. — The growth is slight along the line of inoculation. At the 

 surface it is more noticeable, and it soon begins to liquefy the gelatin. In the 

 liquefaction of the gelatin it has the peculiarity of extending over the entire surface 

 of the tube, but does not extend down the line of inoculation. After liquefying a 

 third the distance down the tube, its growth is checked and it remains apparently 

 inactive, yet after some time the liquefaction extends to the bottom. Quite early in 

 its development a scum forms on the surface of the tube. 



Agar inclined tube-cultures. — It spreads over the surface of the agar in a translucent 

 and blister-like mass. From this, after a time, cream-white spots arise. 



Bouillon- cultures. — A w T rinkled scum forms on surface; the liquid beneath is almost 

 clear and there is no sediment at the bottom. 



Milk cultures . — Milk is first curdled, then peptonized slowly but completely. 



Potato tufa cultures. — Blisters resembling water blisters extend over surface. These 

 eventually dry down into a granular mass which finely becomes wrinkled and of a 

 dark brown color. 



Blood scrum cultures. — Serum is completely peptonized. 



Growth. — Rapid. 



Pigment. — None. 



Oxygen conditions. —Is almost an obligate aerobic bacillus. 



Odor. — The odor of rotting potatoes. 



Acids. — No acid produced in any of the cultures. Upon the addition of Lactose 

 (milk sugar) there was no formation of acid. 



Behavior to gelatin. — Liquefies. 



Gas production. — None observed even in the presence of carbohydrates. 



No. 6. 



(Source. — Taken from Pasteurized milk which has not been recorded in this bulletin. 



Form Hud grouping.— A bacillus. Its thickness varies from f m to 1 m; and its 

 length from '.i m to 7 m. It has square ends an. I is usually arranged in short threads. 



Spores.- Forms median spores in old cultures. 



Protoplasm.- Bomogeneous even to the time of sporulation. Sporogenic granules 

 are not so prominent as many times noticed. 



Motion. — It is sluggishly motile. Its movement is that of a snake. 



Staining reaction. — Stains readily with ordinary aniline stains. 



Note.— m stands for (Jreek letter mu or micron, which is about 1-25.000 of a,n inch. 



