220 STATE BOARD OF AGRICULTURE. 



Potato cultures. — Raised mealy white growth which is not inclined to spread. In 

 this respect, it resembles very closely Bacillus Subtilis. 



Milk cultures. — Curdles milk in a very short time, then peptonizes it rapidly. 



Blood serum cultures. — Completely peptonizes the serum. 



Growth. — Very rapid. 



Pigment. — None. • 



Oxygen conditions. — It seems to grow as readily anaerobically as aerobically. 



Odor. — No odor perceptible. 



Acids. — No acid produced in any of the cultures, not even when lactose or glucose 

 was added. 



Behavior to gelatin. — Liquefies the gelatin very rapidly. 



Gas production. — No gas formation observed. 



No. 29. 



Source. — Was obtained from Pasteurized milk E. 



Form and grouping. — Its thickness varies from i m to ! m, its length from 2 m to 

 7 m. The ends are pointed, and the bacilli usually occur in threads of three and four. 



Spores. — No spores were observed. 



Protoplasm. — The protoplasm is homogeneous and the lines of fission are very 

 distinct. 



Motion. — Very actively motile. It has a progressive tumbling motion. 



Staining reaction. —Stains readily with ordinary aniline stains. 



Temperature. — Withstands 80° C. for 20 min., but is killed at 85° C. for the same 

 time. Its best temperature for growth is 30°-32° C. Below 20° C. it grows very 

 slowly. 



Colonies. — The first stage in the development of the colony is a glistening, granular 

 and regularly formed body. As it grows larger, fine and interwoven threads are 

 noticed to proceed from the border extending to some distance into the gelatin. 

 Tne body of the colony does not verge gradually into the threads but retains its 

 original and definite shape intact through all of its stages of development. The 

 bright glistening appearance remains and the delicate thread like shoots are almost 

 imperceptible. 



Gelatin tube- cultures. — The growth is along the entire line of inoculation, simply a 

 streak at first, with perhaps a slightly heavier growth at the surface. The streak 

 gradually widens out into a funnel shape and extends to the bottom of the tube. 

 The growth is a cloudy path through the gelatin and although it appears as liquefied, 

 there is no liquefaction present. 



Agar inclined tube -cull are. — Almost an invisible sheath covers the surface of the 

 agar along the streak of inoculation. It is translucent, crystalline and moist. There 

 is no spreading over the surface. Isolated colonies may be seen along the streak. 



Bouillon cultures. — A homogeneous clouded appearance with a small amount of sedi- 

 ment at the bottom. Does not clear up after several days. 



Milk rnltnrcs.- -Produces an acid but does not change the appearance of the milk. 

 It seems to remain unaffected. 



Blood s< rum cultures. — No change in the serum. 



Growth. — Rapid. 



Pigment. — None. 



Oxygen conditions, — Grows anaerobically nearly as well as aerobically. 



Odor. — None. 



Acids. — Crown in the presence of lactose, an acid is produced. In litmus-lactose 

 gelatin the litmus is reddened from the surface downward along the growth. 



Behavior to gelatin. — Does not liquefy gelatin. 



,No. 30. 



Source. — Pasteurized milk F, H, J, M. 



Form and grouping. — Bacillus. Its thickness varies from imtolm and its length 

 from 2 m to 7 m. Has square ends and is usually single. 



Spores. — Median spores are formed. They are quickly set free from the parent 

 cell. 



Protoplasm. — Protoplasm maintains a marked homogeneity to time of sporulation. 



Motion. — A tumbling progressive movement. Quite rapid. 



Staining reaction. — Stains readily with ordinary aniline stains. 



