222 STATE BOARD OF AGRICULTURE. 



Oxygen conditions. — Grows much better in the presence of oxygen. 



Odor. — Rotten potato odor. 



Acids. — Acid is not produced in any of the cultures— not in the presence of lactose. 



Behavior to gelatin. — Liquefies gelatin very rapidly. 



NO. 38. 



Source. — Pasteurized milk I. 



Form and grouping. — Bacillus. Its thickness varies from I'm to 1} m; its length 

 from 4 m to 10 m. It appears individually and in short threads. 



Spores. — Spores are rather slow in developing but median spores appear in an old 

 culture. 



Protoplasm. — There is no tendency for the protoplasm to become granular. 



Motion. — Very actively motile. Has a progressive snake like motion. 



Staining reaction. — Stains readily with ordinary aniline stains. 



Temperature.— Grows best at 28° 0-30° C. Withstands 85° C. for 20 min.. but is 

 killed at 90° C. for the same time. Grows very slowly indeed below 20° C. 



Colonies.— Their first appearance is in yellowish-brown pin-head dots. They are 

 crystalline and bright, with a regular border. Soon they send out root-like shoots 

 from the border which make their way through the gelatin in rapid growth. Each 

 shoot has a knotted aspect, and although they are quite distinct a.distance from the 

 colony, yet near the body of the colony they are considerably matted. The regular 

 edge of the original colony is comnk'tely lost. The color has become very dark, 

 almost black. Liquefaction is hardly visible. 



Gelatin tube-cidtu res. —The growth is almost whoLly at the surface, spreading out 

 upon the surface for a short distance. Just beneath the surface, along the line of 

 inoculation, it sends out branches. The development at he surface and the branches 

 continue to increase until the inverted pine tree appears. The only liquefaction 

 that occurs is directly underneath th" colony and that is very limited; only an 

 indentation of the gelatin is it to be notic; >d. 



Agar inclined tube-cultures.- A grayish-white film spreads over the surface. It is 

 moist and almost transparent. 



Bouillon culture — There is a dense wrinkled scum on the surface; below, the 

 liquid is clear. N > sediment appears at the bottom. 



Milk cultures.. — Milk is completely peptonized, becoming watei y. 



Potato tube-cultures. — There is a grayish-white blister-like growih which dries down 

 into an irregular mass. 



Blood serum cultures. — Serum is completely peptonized. 



Growth. — Slow. 



Pigment. — None. 



Oxygen conditions.— Can adapt itself to anaerobic conditirns, but grows best in pres- 

 ence of oxygen. 



Acids. — No acids produced even in the presence >? actose or glucose. 



Behavior to gelatin. — Liquefies gelatin very slowly. 



Remarks. — Some trouble was experienced in making this bacillus grow upon media 

 which seemed very suitable for the others. 



No. 43. 



Source. — Pasteurized milk •/. 



Form and grouping. — Bacillus. The thickness of this bacillus varies from i m to 

 1 m; its length from 2 m to 7 m. Its ends are . quare. The bacilli appear single or 

 in pairs. 



Sjyores. — Median spores are formed. 



Protoplasm. — The protoplasm is homogeneous. 



Motion. — It has a tumbling and progressive motion. 



Staining reaction. — The ordinary aniline stains act readily. 



Temperature.— "Withstands 93° C. for 20 min., but is killed at 96° C. for the same 

 time. Its optimum temperature Ls 25° C -29° C. Grows very slowly below 20° C. 



Colonies. — The colony begins as an irregular mass of a very dark color as though 

 the bacteria were massed Ln a heap. Fine threads soon begin to radiate from this 

 mass and continue to grow till they form a complete whorl. The threads are inter- 

 woven with each other and are of a very light crystalline appearance. Liquefaction 

 takes place so slowly that it is not noticeable in the plates. 



