EXPERIMENT STATION BULLETINS. 225 



Bouillon cultures. — Granular patches are found on the surface. The body of the 

 bouillon is evenly clouded, and there is some fine sediment found at the bottom of 

 the tube. 



Milk cultures. — Milk is peptonized very rapidly. 



Potato tube-cultures. — A yellowish-white growth is obtained which eventually dries 

 into a wrinkled mass. 



Blood serum cultures. — Serum is completely peptonized. 



Pigment. — None . 



Growth. — Rapid. 



Oxygen conditions. — Oxygen is required for growth. 



Odor. — An old musty odor. 



Acids. — No acid is produced even in the presence of lactose or glucose. 



Behavior to gelatin. — Liquefies very rapidly. 



No. 48. 



Source. — This micro-organism was found in sample of Pasteurized milk marked L. 



Form and grouping. — A bacillus whose thickness varies from i m to i m, and whose 

 length varies from f m to If m. It is found single and in pairs. 



Spores. — No spores have been observed. 



Protoplasm. — The protoplasm seemed granular in old cultures. 



Motion. — No decided motion could be discerned; Brownian motion was present. 



Staining reaction. — Stains readily with ordinary aniline stains. 



Temperature. — Its optimum temperature is 30° C.-32° C, but below 22° C. it 

 grows very slowly. It remains alive at 80° C. for 20 min., but is killed at 85° C. for 

 the same time. 



Colonies. —The colonies at first appear as little dots which are crystalline and bright 

 and have a very well denned border. Later there extends from this border a thin 

 plaque over the surface of the gelatin. It is of almost transparent lightness and is 

 •crystalline as the original colony. There is a distinct and even border to this plaque. 

 Th t colony as a whole has a yellowish tinge. 



Gelatin tube-cultures. — The growth extends along the line of puncture, but is heavier 

 •at the surface than deep in the tube; yet a heavy growth develops along the entire 

 line of puncture, and there is no liquefaction whatever. 



Agar inclined tube-cultures. — Is confined to the streak. It does not form a heavy 

 growth and its color is a light lemon yellow. 



Bouillon cultures. — There is an evenly clouded appearance which does not clear. 

 At the bottom is a granular sediment, and at the surface no scum forms. 



Milk cultures. — The milk is unchanged in appearance and is acid. 



Potato tube-culture. — A lemon yellow growth spreads over the surface of the potato 

 but is not exuberant. 



Blood serum-zuUure. — No change in the serum is apparent. 



Growth.— Rapid. 



Pigment. — A lemon yellow pigment is produced. 



Oxygen conditions.— Grows bettar in the presence of oxygen and will grow well 

 under anaerobic conditions. 



Odor. — No marked odor. 



Acids. — Traces of aci ^s produced in the presence of lactose. 



Behavior to gelatin. — Ge'atin is not liquefied. 



NO. 60. 



Source. — Sample of Pasteurized milk O. 



Form and grouping. — Bacillus whose thickness varies from & m to i m and whose 

 length from 1 m to 2 m. The ends are square. They are usually found in short 

 threads. 



Spores. — No spores observed. 



Protoplasm. — The protoplasm soon becomes granular. Whether this has any 

 significance in the formation of spores we are unable to say. 



Motion. — Only Brownian motion present. 



Staining reaction. — Stains readily with the ordinary aniline stains. 



Temperature. — Its optimum temperature is 25° C.-30 C, and it grows very slowly 

 below 20° C. 80° C. for 20 min. does not kill, but it succumbs to 85° G. for theasame 

 time. 



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