226 STATE BOARD OF AGRICULTURE. 



Colonies. — It grows into a colony which is brownish yellow and granular. The 

 borders are regular and sharp. Round in shape and well defined and abrupt borders 

 give the colonies a globular appearance. The colony rests upon and rises above the 

 surface of the gelatin. No sinking or growing into the gelatin occurs. 



Gelatin tube cultures. — The growth extends along the line of inoculation in a heavy 

 line without any liquefaction of the gelatin. 



Agar inclined tube-cultures. — A moist growth of a creamy consistency follows the line 

 of inoculation upon the surface of the agar. Patches of a denser and whiter growth 

 are found upon the surface of the other growth. 



Bouillon cultures. — Avery light scum forms on the surface. The liquid remains 

 cloudy and a sediment is found at the bottom of the tube. 



Milk cultures. — The milk is curdled only. 



Potato tube-cultures. — A slight white and mealy growth appears scattered over the 

 surface of the potato in small patches. 



Growth. — Grows very slowly. 



Pigment. — There is a perceptible yellowish tinge. 



Oxygen conditions. — Grows more readily in the presence of oxygen. 



Odor. — No odor. 



Acids, — Produces an acid in the presence of lactose. 



Behavior to gelatin. — There is no liquefaction. 



No. 62. 



Source. — Taken from Pasteurized sample Z. 



Form and grouping. — Bacillus. Its thickness varies from 1 m to li m in thickness, 

 and from 2 m to 3 m in length. Its ends are tapering. It is found single but some- 

 times in pairs. 



Spores. — No spores were observed. 



Protoplasm. — The protoplasm is homogeneous. 



Motion. — It is not actively motile, but when moving its motion is of a tumbling 

 nature. 



Staining reaction. — The ordinary aniline stains act readily. 



Temperature. — The optimum temperature is placed at 28° C.-32° C, and it grows 

 very slowly below 20°C. 70°C for 20 min. does not kill it, but 75°C for the same time 

 does. 



Colonies.— The colonies are bright and glistening, with borders regular and abrupt. 

 The color is yellowish, yet it is changeable to the different reflections. The center 

 is usually lighter than the border. No liquefaction takes place. 



Gelatin tube-cultures. — A white growth appears along the line of inoculation and 

 does not spread over the surface. 



Agar inclined tubes. — The growth is hardly perceptible ; what is seen is scattered 

 over the surface in very small and scanty patches. 



Bouillon cultures. — A very fine cloudiness pervades the liquid. There is no scum on 

 the surface and no sediment on the bottom. 



Milk cultures. — No change observed. 



Potato tube-cultures. — There is no visible growth. 



Growth.— -Very slow. 



Pigment. — No pigment. 



Oxygen conditions. — Grows anaerobically and aerobically. 



Odor. — No odor. 



Acids. — Simply a slight trace of acid formation in the presence of lactose. 



Behavior to gelatin. — No liquefaction. 



No. 107. 



Source. — Taken from changed Pasteurized milk F. 



Form and grouping. — Bacillus, the thickness of which varies from li m to If m, 

 and the length of which varies from 3 m to 8 m. It is found single and in short 

 threads. 



Spores. — Median spores are formed in old cultures, especially on agar. 



Protoplasm. — The protoplasm is granular. 



Motion. — The motion is progressive but not rapid. 



Staining reaction. — It reacts to the ordinary aniline stains very readily. 



