228 



STATE BOARD OF AGRICULTURE. 



NO. 115. 



Source. — Taken from soured Pasteurized milk I. 



Form and grouping. — Bacillus, the thickness of which varies from li m to 2 m, and 

 the length from 3 m to 12 m. It is found single and in pairs, sometimes in short 

 threads. The ends are round. 



Spores. — Median spores are formed which seem considerably smaller in diameter 

 than the thickness of the cell. 



Protoplasm and cell-wall. — It possesses a granular protoplasm. The cell divisions 

 are very distinct. 



Motion. — There is a progressive serpentine motion. 



Temperature. — Its optimum is 28° C.-32 C. Does not grow below 15° C. It with- 

 stands 80° C. for 20 min., but is killed at 85° C. for the same time. 



Colonies. — The colonies have a very dark center from which radiate lash-like fila- 

 ments. As it continues to grow, there are fine processes proceeding from the center 

 also filling in the spaces between the lash-like processes. The latter resemble the 

 "ray crown" somewhat. However the large limbs bar out the perfect "ray crown.' - 

 Liquefaction begins soon after the fine processes start. 



Gelatin tube-cultures. — The growth is along the line of inoculation, developing deep 

 in the tube as well as at the surface. Liquefaction soon begins and it takes place as 

 fast in the lower portion of the tube as at the upper. This continues till all the 

 gelatin is liquefied. 



Agar inclined tube-cultures. — A heavy, moist and wrinkled growth spreads over the 

 surface. The borders are irregular. 



Bouillon cultures. — No growth on the surface. The cloudiness has a somewhat 

 granular appearance. Sediment is found at the bottom. 



MUk cultures. — The milk is peptonized very rapidly and is of a dark color. 



Potato tube-cultures. — A dirty white and uneven growth spreads over the surface of 

 the potato. 



Growth . — Rapid. 



Pigment. — None. 



Oxygen conditions. — Will grow without free oxygen. 



Acids. — No acid produced even in the presence of lactose and glucose. 



Beliavior to gelatin. — Liquefies gelatin rapidly. 



No. 116. 



Source. — Taken from soured Pasteurized milk J. 



Form and qrouping. — Bacillus. Its thickness varie? from 11 m to 2£ m, and its 

 length from 2£ m to 6$ m. This bacillus forms in short threads. Involution forms 

 are very common in old cultures. 



Spores. — Median spores are formed. 



Protoplasm. — The protoplasm is homogeneous. 



Motion. — It has progressive motion, snake like. 



Staining reaction. — Aniline stains react readily. 



Temperature. — The optimum temperature varies from 22° C.-28° C. Grows very 

 slowly below 15°. It withstands 85° C. for 20 min., but is killed at 90° C. for the same 

 time. 



Colonies. — They begin as light brown spots with well defined borders. Soon fine 

 filaments are seen to proceed from the border and reach out into the gelatin. After 

 a time these filaments become numerous and completely enshroud the body of the 

 colony. The border, however, remains distinct throughout these filamentous 

 changes. The colonies liquefy the gelatin over slowly. 



Gelatin tube-cultures. — The growth is along the entire line of puncture but more 

 abundant at the surface. Liquefaction begins at the upper portion and gives rise to 

 a broad, shallow cup, at the lower portion of which the liquefaction continues along 

 the puncture to the bottom of the tube in a narrow funnel. From this stage the 

 liquefaction continues until the entire gelatin is liquefied. 



Agar inclined tube-cultures. — A moist, heavy and wrinkled growth spreads over the 

 surface of the gelatin. The color is a brownish white. 



Bouillon cultures. — No growth is formed on the surface. The cloudy liquid, instead 

 of clearing, remains impregnated with a flocculent substance. At the bottom is a 

 sediment. - 



Milk cultures. — The milk is rapidly peptonized. Instead of leaving a clear fluid, it 

 is tilled with a flocculent substance. 



