210 ANNUAL REPORT OF THE Off. Doc. 



several classes according to the conditions under which the experi- 

 ments were carried on. The first class includes those cases where 

 the etleci of inoculation was brought out in a striking manner owing 

 to the entire absence of the bacteria in the soil previous to using 

 cultures. A few reports only can be cited and in most cases those 

 selected have been recently received, thus furnishing evidence not 

 only as to the stand secured but also as to the ability of the plants 

 inoculated to go through the winter. The' importance of selecting 

 reports of this kind may be seen from the following, from South 

 Haven. Michigan: 



"Last year I tried a culture of nitrogen-fixing organisms for alfalfa. I reported 

 failure, but this year the difference between the inoculated and uninoculated 

 plots can be plainly seen. The plants inoculated are at least twice as large 

 and much darker in color. I received the inoculation outfit when the plants 

 were about two inches high and sprinkled a small plot in the field with the 

 culture solution. On account of weeds we had to clip real often last year and 

 so we could not see the difference." 



Also a report from Allentown, New Jersey: 



"Good growth last fall, went through the winter fine, coming on fast now. 

 Planted alfalfa on same ground before without treating seed and it proved a 

 failure, but this is a great success." 



From New London, Ohio: 



"Plot sown without inoculation came up good, but did not winter. Plot 

 inoculated wintered and will harvest a fine crop of hay. Sown on clay loam 

 tiled.' 



From Gretna, Louisiana: 



"Of the 100 pounds of seed about two-thirds was inoculated (it being all the 

 bacteria would admit of), and this amount was planted to six acres on Decem- 

 ber 1 and 2. The remainder of seed not inoculated was seeded to remaining 

 four of ten-acre plot. That inoculated grew thick and at this date (April 28, 

 1905,) is 19 to 24 inches high, balance thin and not more than from 6 to 10 

 inches." 



From Doe Kun, Pennsylvania: 



"My alfalfa has a very fine set at present. This is my third attempt, but 

 the first inoculation. The other two were failures. The tops are already 

 (May 1, 1905,) eight inches high and roots twelve inches deep." (Sown last 

 August.) 



From Woodbine, Pennsylvania: 



"Result of inoculation very gratifying. At this date (May 3, 1905,) the plants 

 average fully ten inches in height and stand very thick and of good color. 

 The plants from inoculated seed are fully seventy-five per cent, larger than 

 from untreated seed." 



From Decatur, Georgia: 



"I desire to say that I have had very interesting results here in Georgia with 

 a test of inoculating material on alfalfa. My strip of uninoculated alfalfa 

 that had a good supply of both lime and phosphoric acid is now very yellow 

 and will almost certainly die. All strips of inoculated alfalfa are dark green, 

 but the strip supplied with lime and phosphoric acid has made the best growth. 



