No. 7. 



DEPARTMENT OF AGRICULTURE. 



103 



expenses, |4,554,46; for the enforcement of the law requiring the 

 inspection of cattle shipped from other states, |2,670.65. 



THE LABORATORY. The laboratory has, unfortunately, lost 

 the services of Dr. Samuel H. Gilliland, who has been connected 

 Avith it for a number of years and who has been in charge of the 

 production of biologic products and of the diagnosis of specimens 

 sent in since the resignation of Ur.^ M. P. Ravenel. Dr. Gilliland 

 has also been closely associated with the work upon the immuniza- 

 tion of cattle against tuberculosis. He has taken a very important 

 part in the conduct of the incomplete investigations of the State 

 Livestock Sanitary Board in this field. The necessity for his de- 

 parture on account of ill health is very much regretted. 



During the year the laboratory made and sent out 19,880 c.c of 

 tuberculin, 722 doses of anthrax vaccine and 3,(599 doses of mallein. 

 A large number of specimens were sent to the laboratory for diagno- 

 sis, including the following: 



o 

 B 



Rabies, 



Anthrax, .... 

 Tuberculosis, 

 Glanders, . . . 



80 



2 



19 



41 



15 



12 

 14 

 16 



97 

 14 

 32 

 57 



There were also submitted a large number of samples of milk, 

 tumors, parasites and of pathological material. ^ 



Much work has been done to establish the reliablity of the method 

 generally used in the rapid diagnosis of rabies, as is explained under 

 the section in relation to that disease. The laboratory diagnosis 

 of glanders has usually depended upon the method of Strauss. 

 In addition to this method, the agglutination test was made with 

 blood serum from 24 horses. The serum of 8 horses aggluntinated 

 the baccilli of glanders in the dilution of 1-1000 and over. Several 

 showed a well marked agglutination in dilution of 1-2000. The 

 serum from 16 horses that were free from glanders, caused an 

 agglutination in dilution of more than 1-500 in but one instance, 

 the average being 1-300. The exception caused a distinct agglutina- 

 tion in a dilution from 1-GOO; in this case the serum was taken from 

 the blood of a horse suffering with acute lymphangitis. All of the 

 work in relation to the diagnosis of rabies and glanders has been 

 carried on by Dr. John Reichel. The result of his work in regard to 

 the aggluination test for glanders indicates that this method is 

 destined to be of service in connection with the control of this 

 disease. 



The experimental work carried on at the farm has been under 

 the care of Dr. E. S. Deubler, who has also conducted some 

 of the work of the laboratory. Several important lines of research 

 have been conducted that Drs. Deubler and Reichel who will re- 

 port through other channels. Dr. C. Y. White continues patholo- 

 gist to the laboratory. A large amount of pathological work has 



