No. 7. DEPARTMENT OF AGRICULTURE. 115 



thetic ganglia were examined in many cases to determine their val- 

 ue in the diagnosis of rabies by the presence or absence of the pro- 

 liferation changes. The changes in the sympathetic ganglia were 

 found to be more constant than the changes in the medulla oblon- 

 gata but, like the changes in the gasserian ganglia, they were also 

 found in several cases shown not rabid by experimental animal in- 

 oculation and by the absence of Negri bodies. 



"Negri bodies were sought for in 126 cases, they were found in 99 

 and absent in 27. Where the Negri bodies were not found, experi- 

 mental animal inoculations were always made, and it w^as learned 

 that in two cases rabies developed in the experimental animals in- 

 oculated, although Negri bodies were not found in the nervous sys- 

 tem from which the experimental animals were inoculated. The 

 history of these animals, however, shows that both were killed early 

 in the course of the disease. 



"Of the many negative cases examined, including the 27 negative 

 suspected cases and the nervous systems of healthy animals and 

 animals dead of the diseases, Negri bodies have not been found 

 in a single case. 



"The hippocamjms major usually shows the Negri bodies in the 

 greatest number but they are just as constantly present in the 

 cerebellum wiien none were found in hippocampus major. Although 

 the hippocampus major and cerebellum are the usual structure ex- 

 amined for Negri bodies, they have been found in the olfactory lobes 

 (quite numerous) cerebral cortex (not constant); medulla oblongata, 

 gasserian and sympathetic ganglia and in the cervical region of the 

 spinal cord. 



"Little work has been done in studying the nature of the Negri 

 bodies outside of staining them and perfecting the method by which 

 they can be demonstrated in the quickest way. 



"The smear method, published by Drs. Anna Williams and May 

 Murray Lowden of the New York Board of Health, appears to be the 

 most accurate and quickest way to demonstrate the bodies so far 

 proposed. In no case where the Negri bodies were not found by 

 the use of the smear method were thay later found in sections made 

 and stained from the various structures usually examined. By 

 the use of this method, Negri bodies have been demonstrated in from 

 15 to 30 minutes. 



"To permit a suspected rabid animal to live and develop observa- 

 ble clinical symptoms is quite as important in the making of a diag- 

 nosis in the examination for Negri bodies as it has previously been 

 shown when the proliferation changes were alone sought for. That 

 the Negri bodies may be foimd earlier than the proliferation changes 

 in the gasserian ganglion, may be true, but neither is found much 

 earlier than the appearance of clinical symptoms." 



HOG CHOLERA. -Hog cholera occurred durinn: the past year in 

 the following counties: Armstrong, Bedford, Blair, Berks, Brad- 

 ford, Bucks, Centre, Columbia, Cumberland, Dauphin. ForesL Frank- 

 lin, Lancaster, Lebanon, Jefferson, Luzerne, Lycoming, McKean, 

 Montgomery, Perry, Susquehanna, Union, Venango, Westmoreland 

 and York. The disease was not widely scattered in the counties in 

 which it occurred excepting in Cumberland county^ where 66 farms 



