432 STATE BOARD OF AGRICULTURE 



ORGANISMS ISOLATED 



Bacillus I A 

 I. Morphology: 



1. Vegetative cells. 



Slender rods with rounded ends. Occur singly and in short 

 chains. 



2. Endospores. 



Position — near one end. 



Form — oval. 



Spores were found in broth. 



3. Motility. 



Actively motile. 



4. Staining. 



Stains well with methylen blue. 

 Gram negative. 



II. Cultural Characteristics: 



1. Agar Slant. 



Growth scanty, filiform, slightly raised, glistening, smooth, 

 translucent, white, odorless, slimy. 



2. Gelatin stab : 



Growth best at top; beaded; liquefaction saccate, becoming 

 stratiform, liquefied part clear. 



3. Broth culture: 



Wrinkled, membranous surface growth, slimy; no clouding. 



4. Potato Culture: 



Abundant growth in form of ''water blisters" or blebs. 

 Slimy, slightly pink tinge. 



5. Litmus milk. 



Slimy, neutral, curded. Peptonization begins in six days. 



III. Physical and biochemical features: 



1. Temperature relations. 



Grows best within the temperature range of 37°-55°C, but also 

 grows well at 20°C. Killed at 110°C. for 10 minutes. 



Bacillus I B 

 I. Morphology: 



1. Vegetative Cells: 



Slender rods occurring singly and in filaments. 



2. Endospores : 



Position — ^near one end. 



Form — oval. 



Spores found in broth culture and agar slant. 



