EXPERIMENT STATION BULLETINS. 463 



3. Motility: 



Actively motile. 



4. Staining: 



Stains well with methylen blue. Gram negative. 



II. Cultural Characteristics: 



1. Agar slant: 



Growth scanty, filiform, raised, glistening, smooth, translucent, 

 white, odorless, slimy. 



2. Gelatin stab. 



Growth best at top. Liquefaction saccate and stratiform. 



3. Broth Culture: 



White, wrinkled, slimy membrane over top of broth. 



4. Potato Culture: 



Transparent blebs, becoming opaque. Slimj', grayish-white. 



5. Litmus milk. 



SUmy, neutral curd. Much peptonization in six days. 



III. Physical and biochemical features: 



1. Temperature relations: 



Optimum temperature range 37°-55°C. Grows well at 20°C. 

 Killed by heating at 80° ten minutes. 



Bacillus IV A 

 I. Morphologj^: 



1. Vegetative cells: 



Slender rods occurring singly and in chains. 



2. Endospores : 



Position — ^near one end. 

 Form — oval. 



3. Motility: 



Motile. 



4. Staining: 



Stains well with methylen blue. Gram negative. 



II. Cultural Characteristics: 



1. Agar stab: 



Growth abundant, filiform, raised, glistening, smooth, trans- 

 lucent, creamy white, slimy. 



2. Gelatin stab: 



Growth best at top, beaded. Liquefaction — crateriform be- 

 coming stratiform. Begins in 3 days. 



3. Broth Culture: 



Wrinkled, slimy, white membrane. 



4. Potato Culture: 



Growth abundant, beaded, raised, cretaceous, slimy. 



5. Litmus milk: 



Slimy, neutral, and slow peptonization, 



