464 STATE BOARD OF AGRICULTURE 



III. Physical and Biochemical Features: 



1. Temperature relations: 



Optimum temperature 37°-55°C. Grows at 20°C. 



Bacillus VI B 

 I. Morphology: 



1. Vegetative cells: 



Rods occurring singly and in chains. • 



2. Endospores : 



Position — near one end. 

 Form — oval. 



3. Motility: 



Actively motile. 



4. Staining: 



Stains well with methylen blue. Gram negative. 



II. Cultural Characteristics: 



1. Agar slant: 



Growth scanty, filiform, flat, glistening, smooth, translucent. 



2. Gelatin stab: 



Growth best at top. Liquefaction — whole tube softened. 



3. Broth culture: 



Slimy membranous surface growth. 



4. Potato culture: 



Moderate growth in form of water blisters or blebs. Trans- 

 parent at first, becoming opaque. SKmy. 



5. Litmus milk: 



Litmus reduced in four days. 



III. Physical and Biochemical Features: 



1. Temperature relations: 



Optimum temperature range 37°-55°C. Grows well at 20°C. 

 Killed by exposure at 90°C for ten minutes. 



Bacillus VII B 



I. Morphology: 



L Vegetative cells. Rods single and in chains. Occasional long 

 filaments. 



2. Endospores : 



Position — near end. 

 Form — oval. 



3. Motility: 



Actively motile. 



4. Staining: 



Stains well with methylen blue. Gram negative. 



