EXPERIMENT STATION BULLETINS. " 465 



II. Cultural Characteristics: 



1. Agar Slant: 



Growth abundant, filiform, flat, glistening, smooth, trans- 

 lucent, shmy. 



2. Gelatin stab. 



Growth plumose. Distinct star-shaped colonies. No 

 liquefaction. 



3. Broth Culture: 



Slimy membranous growth. 



4. Potato Culture: 



Abundant, spreading, ghstening, smooth, white, slimy growth. 



5. Litmus milk: 



Slimy neutral curd. 

 Slow peptonization. 



III. Phj^sical and Biochemical Features: 



1. Temperature relations: 



Optimum temperature, 37°-55°C. Grows well at 20°C. 

 Killed by exposure to a temperature of 100°C for 10 minutes. 



Bacillus VIII A 

 I. Morphology: 



1. Vegetative cells: 



Slender rods occurring singly and in filaments. 



2. Endospores: 



Position — near one end. 

 Form — oval. 



3. Motility: 



Motile. 



4. Staining: 



Stains well with methylen blue. Gram negative. 



II. Cultural Characteristics: 



1. Agar slant: 



Growth moderate, filiform, raised, glistening, contoured, 

 translucent, creamy, slimy. 



2. Gelatin stab: 



Growth best at top, beaded, liquefaction saccate, becoming 

 stratiform, begins in three days. 



3. Broth culture: 



Wrinkled, white, slimy, membranous surface growth. 



4. Potato culture: 



Transparent slimy blebs becoming opaque in three days. 



5. Litmus milk: 



Slimy, rennet curd. Slow peptonization. 



III. Physical and Biochemical Features: 



1. Temperature relations: 



Optimum temperature range 37°-'55°C. Grows well at 20°C. 



