EXPERIMENT STATION BULLETINS. 395 



c.c defibrinated blood from Expt. pig 323 B mixed with 100 c.c. of a 

 10 hour bouillon culture of B. eholerae suis. 



On l-4-'10 there was injected 150 c.c. of defibrinated blood from Expt 

 pig 255 mixed with 150 c.c. of an 18 hour bouillon culture of B. eholerae 

 suis. 



Pig thin but vigorous and good appetite; walks with stilted gait. 

 No abscesses visible or palpable. 



On 1-12-'10 drew 289 c.c. blood from tail, defibrinated and preserved 

 in .5% phenol. 



On l-]3-'10 injected 150 c.c. defibrinated blood from Expt. 193 mixed 

 with 150 c.c. of a 16 hour bouillon culture of B. eholerae suis. 



On the afternoon of this day the pig died. The only lesions were 

 local and consisted in an infiltration and necrosis of the muscular and 

 connective tissue between the shoulder and thorax. The tissues were 

 greenish in color, spongy and saturated with a foul-smelling, dirty 

 liquid. The last injection did not appear to have been absorbed. 



An account should be given of the pigs furnishing the blood with 

 which the cultures were mixed. 



Expt. 237, Yorkshire, wt. 29 lbs. 7-8-'09, received 10 c.c. mixed serum 

 264 and 1 c.c. virus Expt. 101. Kemained well. 12-21-'09 killed; normal. 



Expt. 323 B., wt. 36 lbs. Just received. No treatment. 12-28-'09, 

 killed; normal. 



Expt. 255, wt. 21 lbs. 7-8-09, received 12.5 c.c. mixed serum 28. 12- 

 3-'10, killed ; normal. 



Expt. 193, wt. 15 lbs. 4-29-'09, received 15 c.c. mixed serum 17 and 

 1 c.c. bouillon culture B. eholerae suis, "No. 2 Rabbit 25" in the ear 

 vein. 5-25-'09 injected qne agar slant, 24 hours old B. eholerae suis 

 "No. 2, Rabbit 25" in muscles. Produced abscess; opened and disin- 

 fected. 



6-22-'09 injected 1 c.c. virus 99 in muscles; 



7-16-'09 injected 5 c.c. virus 107 in muscles; 



l-ll-'lO killed; normal. 



Our object was to make an artificial virus blood containing a maxi- 

 mum of B. eholerae suis but none of the filterable virus. There is no 

 reason to believe that any of the blood used by us contained the filterable 

 virus. 



Virus pig 139 is to be regarded as a pig hyper-immunized to B. 

 eholerae suis. We should expect to find in the blood serum of this pig 

 anti-bodies responsive to the specific antigen, B. eholerae suis but none 

 for the filterable virus (unless there is an intimate biological relation 

 between the two). 



Table I gives the results of a few agglutination tests with the blood 

 of this pig. An increase in the agglutinative power from 1-125 to 1-25000 

 is noted. 



We know of no test capable of disclosing to us anti-bodies for the 

 filterable virus except the biological test, using pigs. We accordingly 

 tested the serum, drawn from virus pig 139 against hog cholera virus 

 by injecting small pigs. The virus used was not filtered and may have 

 contained both the filterable virus and B. eholerae suis. 



Table II shows that the serum from virus pig 139 possessed consider- 

 able potency against hog cholera virus. Small pigs were not protected 

 against 1 c.c. of virus by 10 c.c. or 15 c.c. of this serum but protection 



