EXPERIMENT STATION BULLETINS. 397 



was offered by 20 c.c. and 25 c.c. That the virus used was virulent is 

 shown by the death of Expt. pigs 305 and 3G6 and by the development 

 oi" hog cholera in virus pig 149. 



•Should we conclude that anti-bodies against the filterable virus are 

 produced as a result of using li. cholerae suis for the antigen? The P^x- 

 periment points strongly to the possibility of using cultures of B. 

 cliolerae suis in noruin! pig blood for hyper immunizing serum hogs. 



Possibly the results of this experiment depended partly upon the 

 blood used in connection with the cultures. 



We, accordingly, attempted to hyperimmunize an immune pig with 

 cultures of B. cholerae suis only. For this experiment, we used the same 

 strain employed in the preceding experiment and Expt. pig 315. 



Expt. pig 315, Chester white, weight 37 lbs. 



On ll-30-'09 injected 25 c.c. mixed serum 41 and 1 c.c. virus 128. 

 Pig remained well. 



On 2-l-'10 injected 10 c.c. virus 149. Pig remained well for over three 

 weeks, showing immunity to hog cholera virus. 



On 2-24-'10 injected 10 c.c. of a 24 hour bouillon culture of B. cholerae 

 suis into axillary region. Estimates from agar plates indicated about 

 500 million bacteria per c.c. 



No local lesion could be detected on 3-3-'10 when 45 c.c. of a 24 hour 

 bouillon culture of B. cholerae suis was injected into the axillary 

 region. 



On 3-ll-'10 injected into axillary region 45 c.c of a 24 hour bouillon 

 culture of B. cholerae suis. Platings on agar indicated the presence of 

 from one-half to one billion bacteria per c.c. No visible or palpable 

 lesions locally. 



•On 3-19-'10 injected 40 c.c. of a 24 hour bouillon culture of B. cholerae 

 suis into the axillary region. Platings indicated the presence of about 

 one billion bacteria per c.c. No very distinct swelling locally but con- 

 siderable soreness. Bled 180 c.c. from tail, defibrinated and preserved 

 in 5% carbolic acid, 10 c.c. to 90 c.c. of blood. 



On 4-2-'10 bled from tail 289 c.c, defibrinated and preserved in car- 

 bolic acid. 



On 4-27-'10 bled from tail 400 c.c, defibrinated and preserved in car- 

 bolic acid. Mixed bleedings of 3-19-'10, 4-2-'10, and 4-27-'10. Labeled, 

 "Mixed Serum Expt. 315." 



On 5-20-'10 drew sample from tail for agglutination test. 



On 6-8-'10 drew sample from tail for agglutination test. Pig weighed 

 143 lbs. on 6-24-10. 



Killed, 7-l-'10. In good condition. 



Autopsy: In each axillary region were extensive abscesses, well de- 

 fined and encapsulated with no indication of infiltration into the sur- 

 rounding tissues. Contents of abscesses, a dirty yellowish-brown, thick 

 liquid with foul odor. Inoculations gave B. cholerae suis in pure cul- 

 ture. Other organs, normal. Pieces of spleen, kidney, liver and heart 

 produced no growth in bouillon. 



Table III shows the results of agglutination tests with samples of 

 serum from Expt. pig 315 drawn at various times. There is manifestly 

 a great stimulus to the production of agglutinins. The mixed serum 

 used in the biological test (vide Table IV) give a reaction at 1-500,000. 

 We have previously found that all samples of Dorset-Niles serum 



