EXPERIMENT STATION BULLETINS. 



401 



land candles. One portion clouded, others clear after 2 days incuba- 

 tion, 



Oct. in, injected 70 c.c. virus Expt. 453 to 450. This was one of the 

 portions described above. 



Oct. 26, injected 50 c.c. virus 224 and 200 c.c. virus 227. Prepared 

 as above; virus 224 filtered through one Chainberland filter only. Virus 

 227 filtered through four Cluunberland filters. Both samples incubated 

 and remained clear. 



Nov. 2, bled from tail enough to make 663 c.c. after defibrinating and 

 adding .5 per cent trikresol. Injected 75 c.c. virus 224 and 225 c.c. 

 virus 229. Prepared as above. Virus 224 was a portion of that de- 

 scribed above. Virus 229 filtered through one Berkefeld and 3 Chamber- 

 land filters. Used only first filtrate. 



Nov. 9, killed. Dressed 122 lbs. Secured 1039 c.c. defibrinated blood 

 preserved in .5 per cent trikresol. All the injections had been made 

 in the axillary region. There was never any swelling or soreness. At 

 autopsy, only a yellowish-brown discoloration of the tissues at point 

 of inoculation was discovered. 



The records show that a total of 2820 c.c. of diluted virus was used 

 to hyperimmunize Expt. 387. The pig weighed about 160 lbs. when 

 killed. There was, therefore, used only about 17.5 cc. of diluted virus 

 instead of 20 c.c. per pound live weight. 



Samples of both bleedings were tested for agglutinative power against 

 B. cholerae suis. Table V shows results of agglutination tests, with the 

 tail bleeding and last bleeding of Expt. pig 387. 



TABLE V. 

 Agglutination tests with serum from experiment 387 and B. cholerae suis. 



The agglutinative power of this serum is as great as that of nearly 

 two-thirds of the samples of Dorset-Niles serum prepared in the usual 

 way. Unfortunately, we failed to test the agglutinative power of the 

 blood of this pig before making the filtered vinis injections. We have 

 previously found that pigs treated by the serum-simultaneous method 

 51 



