412 STATE BOARD OF AGRICULTURE. 



Similar experiments by M'Fadyean aud Stockman^ showed a tempera- 

 ture reaction up to 105° lasting three days in one case, and in the other 

 a reacti<m uj* to 100.4° lasting seven days. 



The heifers were bred as follows: Dark, June 27, 1911; Light, June 

 29, 1911, to King Bowser, the college Jersej^ bull. 



Both heifers were brought in from pasture November 7, after an un- 

 eventful summer. 



November 8. drew sample of blood from jugular of each. Agglutina- 

 tion tests with these samples gave the following results: 



Light— 1-10 to 1-250 complete. 

 Dark— 1-10 to 1- 50 complete. 



On the same day there was injected intravenously into each heifer 

 10 c.c. of culture of "MacNeal" in heated horse serum + glucose 

 glvcerine bouillon 5 davs old. 



The following temperature records were made: 



Nov. 8 — night 



Nov. 9 — moniing 



noon 



night 



Nov. 10 — morning 



noon 



night 



Nov. 11^ — morning 



noon 



night 



Nov. 13 — morning 



noon 



night 



Nov. 14 — morning 



noon 



night 



Nov. 15 — morning 



noon 



night 



Nov. IG — morning 



noon 



The tempernturo reaction in these cases was very sliglit. No other 

 effect of the injection was observed. 



On Feb. Ui. 1912, injected into jugular of each heifer 10 c.c. of cul- 

 ture "MacNeal" in amniotic fluid 20 days' growth, also injected .30 c.c. 

 into vagina (if each. The following temperature records were made: 

 Feb. 17. Dark, 1()().2, Liulit, 101.2 



Feb. 18, Dark, 101.0, Light. 101.2 



Feb. 19, Dark, 101.3, Light, 101.4 



