EXPERIMENT STATION BULLETINS. 



435 



TABLE XVIII. 

 Lactone plus Healed and Unhealed FiUrale. 



The stimulation and subsequent dying off of the lactic bacteria occurs 

 in this experiment also. Strain 4 was used in a duplicate experiment 

 with only slightly different results. One cc. of the filtrate was added 

 to each culture. 



TABLE XIX. 

 Strain No. 4 + Heated and Unhealed Filtrate. 



*Too large an initial inoculum. 



♦♦Dilutions too high to obtain counts, 1-1,000,000, 1-5,000,000 and 1-10,000,000 used. 



♦♦♦Dilutions of 1-100, 1-1000, and 1-10,000 used. Lactic bacteria considered dead. 



In Table XVIII, at 5 days the unheated filtrate gave evidence of hav- 

 ing stimulated the lactic, and the curd in this flask also of having been 

 formed for a longer time than in the check flask or in that of Lactone 

 plus the heated filtrate. The acid production showed no stimulation by 

 the use of the filtrate either heated or unheated; the optimum virility 

 was reached earlier in the flasks containing the filtrate. The fact that 

 curding was more marked in the unheated filtrate shows that this phe- 

 nomenon is caused to some extent by an enzyme. The proof of the pres- 

 ence of an enzyme which causes a stimulation of the lactic bacteria is 

 shown more conclusively in Table XX under Expt. III. 



