436 STATE BOARD OF AGRICULTURE. 



Expt III. • • 



The Effect on the Lactic Organism of Varying the Amounts of Heated 



and Unheated Filtrate. 



In this experiment, different amoimts of the filtrate heated and un- 

 heated were added to milk cultures of the lactic organism. Seven milk 

 tlasks containing 250 cc. milk each were inoculated with 1 cc. of a milk 

 culture of 8tock lactic, one of these flasks was reserved for a check 

 and to the others was added respectively 1 cc, 5 cc. and 25 cc. of the 

 heated and unheated filtrate of the yeast LZ. These cultures were 

 j)lated immediately for the initial counts and as soon as the milk curded 

 in any fiask this milk with its duplicate in the lieated filtrate and the 

 check also were titrated and plated; each milk culture as it curded was 

 plated with duplicate as above. 



The milk culture containing the lactic bacterium plus 25 cc. of un- 

 heated filtrate was the first to curd, that containing 5 cc. unheated fil 

 trate next and that containing 1 cc. unheated filtrate last, neither the 

 check culture nor the cultures containing the lactic organism plus the 

 heated filtrate curding at all. The results of this experiment are found 

 in Table XX. 



