486 



STATE BOARD OF AGRICULTURE. 



tion. The whole arrangement corresponds to the experiments of Tables 

 X, XI, XII. Table XIX gives the acidity in degrees, and the numbers 

 per cc. of liquid. 



TABLE XIX. 



The variation in the first two samples is within the limits of error, 

 and it may be stated safely that a fourfold increase of surface has practi- 

 cally no influence upon this strain. In the last column, sand -f 10% 

 milk, the surface is increased approximately 1000 times, and the result 

 is a distinct retardation of growth and acid production, especially in 

 the beginning. The milk-saturated sand was the best medium, because 

 it afl'orded more strictly anaerobic conditions than the milk, and be- 

 sides, the absorption may exercise a helpful inliuence. 



To study the role of the moisture film, two larger exix^riinents were 

 carried out which are really duplicates of the above with coarse, medium 

 and fine sand. The milk in shallow layer was omitted. Since the i)lat- 

 ing and titrating required a good deal of time, each experiment was 

 divided into two sections which were carried one hour apart with 

 separate checks. For all data which are compiled in Tables XX and 

 XXI, I am obliged to Mr. A. McVittie. 



TABLE XX. 

 Strain II of Baderium ladis acidi. 



