322 



A s o, On the Nature of Oxidases. 



Froni tlic above results. I hecuiiu^ oonvincetl tlial amido- 

 coinpounds (locom]iose nitrite in a very f'aint acid Solution and 

 i( is nerossary to niakc tlie .Solution alkalino to preserve the ni- 

 trite. Hence I niade analogous experiments with plantjuices. 



Experiment witli Sajfittaria. 



18 grams of the buds of Sagittaria were crushed, extracted 

 with 100 c. e. water and divided into three äqual parts. To 

 one a few drops of acetic acid, to the other a few drojDs of 

 caustic potash were added while the third served as contiol. 

 Each Solution was lieated to l^oiling for five minutes and tested 

 as mentioned before: 



Control 



Alkaline 

 Solution 



Acid 

 Solution 



Potassiuniiüdifl- 

 starch. reaction 



Griess reaction 

 Guaiac reaction 



Di.srinct 



No reaction 

 at all 



Di.stinct 



'No reaction 

 at all 



No reaction even 



after several 



hours 



faint 



No reaction 

 at all. 



Quito similar a test was repeated, but in tliis case, the So- 

 lutions were lieated to 95 ° C for 10 minutes and filtered after 

 acidification witli acetic acid, wliicli had produced some pre- 

 cipitate: 



Control 



Alkaline 

 Solution 



Acid 

 Solution 



Potassiumiodid- 

 starcli reaction 



No reaction at Distinctly No reaction at first, 

 first, but after , at once Init after 10 min., 

 10min. appeared a reaction appeared 



gradually altliougli weaker than 



in the control case 



Grriess reaction Distinctlv Distinctly i 



Giuüac reaction No reaction ;i1 ;il] No reaction! 



at all I 



Distincly 



No reaction 

 at all. 



sokitioiis were filtered and the liltrates were tested witli the reagents ab- 

 ove-nientioued. Hereupon Griess" and iodine reaction appeared very dis- 

 tinctly, though the latter was a little weaker in boiled liquid than in the 

 ronti'ol: but no blue reaction of guaiac at all appeared. Similar facts were 

 observed repeatedly. 



