A s ö , On tlie Nature of Oxidases. 325 



Chloride. Tlie Griess reaction appeared, bnt only in traces. The 

 main part of the juice (al)out 25 c. c.) was mixed with three 

 times of its vohime of alcohol (90 %) and hltered. The hltrate 

 showed a veiy faint Griess reaction, but no reaction for oxidi- 

 zing enzym.s and also with potassium-iodid-starch, wliile the 

 aqueous Solution of the well-washed precipitate gave very strong 

 reactions for oxidizing enzyms, but not the Griess reaction and 

 also no iodine reaction. 



Experiment witli Pea. 



4 grams of the root of a füll grown peaplant were crushed 

 and extracted with 30 c. c. wather. The juice obtained had al- 

 most a neutral reaction and gave strong reactions for oxidizing 

 enzyms, but neither liberation of jodine nor the Griess reaction. 

 20 grams of the green parts ') of the pea plants were crushed 

 and extracted with 30 c. c. water, and tested in a similar way 

 as above with the same results. 4 grams of the root-tubercles 

 were now crushed, and the pressed juice of a faint acid reaction 

 gave strong reactions for oxidizing enzyms, but no liberation of 

 iodine nor the diphenylamine reaction. Also Fe hl in gs Solution 

 w^as not reduced. Further 5 grams of the white part of the 

 stem of pea-shoots (5 — 10 cm. long) were crushed and extracted 

 with 2 c. c. water. After filtering and adding a few drops of 

 concentrated Solution of basic lead acetate, the iiltrate was mixed 

 with some sulphanilic acid and sulphuric acid and filtered. To 

 the filtrate, a few drops of K-naphthylamine hydrochloride were 

 added, whereupon the Griess reaction appeared feebly, but dis- 

 tinctly. Again, 10 grams of the white part of the pea-stem 

 were crushed and the juice was pressed out. The juice produced 

 strong color reactions for oxidizing enzyms and diphenylamine 

 reaction for nitrate, but neither Griess nor potassium-iodid-starch 

 reaction for nitrite. The juice was now treated with a few drops 

 of concentrated Solution of basic lead acetate and filtered. To 

 one part of the filtrate, some sulphanilic acid and sulphuric acid 

 were added and again filtered. The filtrate showed here again 

 a distinct Griess reaction upon the addition af «-naphthylamine 

 hydrochloride. With 10 grams of the green part of the same 

 shoots, were, after the same treatment, tested in the sanier man- 

 ner. But neither the diphenylamine reaction nor the Griess re- 

 action was here obtained. 



If) grams of Sagittaria shoots (of green color) were crus- 

 hed witli 50 c. c. water and filtered. The filtrate showed very 

 strong reactions for several oxidizing enzyms, but a very weak 

 potassium-iodid-starch reaction.^) This filtrate was diluted with 



1) In tis case, it is necessary to add mucli gxiaiac tincture; otlierwise 

 the blue color soon fades oiit. 



2) After boliing and tiltering, tlie filtrate showed a weak iodine reaction, 

 but no reaction for oxidizing enzyms. 



