ANATOMY OF LYCOPODIUM REFLEXUM^ 



J. Ben Hill 



(with five figures) 



In a former paper,^ giving in some detail the results of an 

 investigation of the development and specialization of the steles 

 of 6 species of Lycopodium, I reviewed the hterature of the subject 

 of the anatomy of Lycopodium. The anatomical studies have 

 emphasized "types" of steles as characteristic of various species of 

 Lycopodium, among which the radial and parallel-banded arrange- 

 ments predominate. In my former paper I suggested that it was 

 inadvisable to regard any stelar arrangement as characteristic of a 

 species of Lycopodium, since almost all "types" may be found 

 in a single species, and even in a single plant at different levels 

 in the stem. 



The plants of L. reflexum used in this investigation were col- 

 lected by Dr. C. R. Barnes and Dr. W. J. G. Land in the vicinity 

 of Xalapa, Mexico, in 1908. The habitat is described as moist 

 clay soil. The material was preserved in a formaldehyde-alcohol 

 solution and was given to me in this condition by Dr. Land, to 

 whom I wish to express my thanks. The slides for the investi- 

 gation were prepared from paraffin serial sections cut transversely 

 10-15 A' i^ thickness and stained in safranin-light green and in 

 iron-alum haematoxylin-safranin, both combinations producing 

 excellent results; the former is slightly better for differentiating 

 protoxylem in sections of young stems, and the latter better for 

 older tissues. 



Investigation 



In a study of the sections of the stem the most important 

 matter of interest is concerned with the so-called "types" of 

 stele to be found in L. reflexum; a secondary significant feature is 



' Contributions from the Department of Botany of the Pennsylvania State 

 College, no. 1 7. 



^ Hill, J. Ben, The anatomy of six epiphytic species of Lycopodium. Box. 

 Gaz. 58:61-85. figs. 28. 1914. 



Botanical Gazette, vol. 68] [226 



