1919] KEMPTON—PYCNIDIUM 235 



Although in many instances the fungi were not studied primarily 

 as to their pycnidial development, and such information as was 

 collected regarding this was incidental, according to the figures and 

 descriptions given the following belong to the meristogenous group 

 of DeBary: Pleospora (Gibelli and Griffini 17; Bauke 5), 

 Cucurbitaria (Eidam 14; Bauke 5), Pycnis (Brefeld 6), Fumago 

 (TuLASNE 43; ZoPF 53; ScHOSTAKOWiTSCH 34), SpJiaeronaema 

 (Halsted and Fairchild 18), pycnidium with Teichospora 

 (Nichols 27), Phyllosticta with AUernaria (Planchon 29), 

 Sphaeropsis and Coiiiothyrium (Potebnia 30), Chaetophoma 

 (Arnaud 3), Phoma (Reddick 31; Mercer 26; Schnegg 33), 

 Endothia (Anderson i). As symphogenous may be classed: 

 Cicinnoholus (DeBary 8), Fmnago- (Tulasne 43; Zopf 52) ,• 

 Diplodia (Bauke 5; Van der Bijl 44), Graphiola (Fischer 15), 

 Cystispora (von Tavel 46), Hendersonia (Voces 45; Wolf 51), 

 pycnidium with Sphaerella (Higgins 21), Sphaeropsis (Hesler 19), 

 Septoria (Levin 25). A few of the species have both types of 

 development. 



The present investigation is an endeavor to extend our knowl- 

 edge of the very early stages in the development of the pycnidium 



and kindred structures. 



Methods 



For the present study, the fungi were either isolated from their 

 hosts or cultures were procured from various sources as noted. 

 Corn meal agar (35) was used because it gave an abundant growth, 

 and its transparency facilitated the study of even the youngest 

 stages. Other media were used in a few instances for comparison. 

 Cultures for study, were grown in Petri dishes, at room temperature 

 (15-37° C.). Transfers were made by lifting hyphae or spores 

 from pure cultures to the poured agar in Petri dishes. Drop cul- 

 tures of melted agar were made by the use of sterile pipettes. In 

 some cases the drop of agar was inoculated with spores as for. dilu- 

 tion plating. In others, each drop was inoculated by transfer. 

 These drop cultures served well for study of early stages of develop- 

 ment, but seldom produced mature conidiiferous structures. 



Disks of agar containing young pycnidia were removed from 

 Petri dish cultures, placed on cover glasses, and inverted on 



