324 



BOTANICAL GAZETTE 



[NOVEMBER 



In general the amino N varies directly with the insoluble protein; 

 with a high protein content, high amino N; and low protein con- 

 tent, low amino N. 



TABLE II 



The nitrogen compounds in the green plant 



Material 



Total N 



Nitrates NOj . . . 



Nitrate N 



Amino N 



Insoluble N 



Insoluble protein 

 Soluble protein. . 



Total N 



Nitrates NO3 . . . 



Nitrate N 



Amino N 



Insoluble N 



Insoluble protein 

 Soluble protein. . 



Total N 



Nitrates NO3 . . . 



Nitrate N 



Amino N 



Insoluble N 



Insoluble protein 

 Soluble protein. . 



Roots 



Stems 



Branches 



Leaves 



June 3 collection (1-4 inches) 



3-33 

 1.77 

 0.40 

 1 .09 

 1 .62 

 10.18 

 8.24 



S-33, 

 1-93 

 0.43 

 I 13 

 152 

 10.25 



8.17 



19 

 99 



22 



43 

 74 



3.21 

 1 .04 

 0.23 



0.43 

 1-73 



10.92 10.80 

 7.67 7.92 



27 

 40 

 32 



96 



23 

 30 



52 



June 20 collection (6-8 inches) 



2-39 

 1 .67 



0.38 

 o. 19 



095 

 5.86 

 5.28 



2. 21 



1-75 

 0.40 

 o. 19 

 0.97 

 6. 10 

 S-4I 



4 56 

 None 

 None 



1.38 

 3-66 



451 



None 



None 



1-37 



3-59 



23 . 00 22.6 

 5.66 578 



July 8 collection (20 inches) 



21 

 41 

 99 

 63 

 29 

 .10 

 16 



3.12 



4-47 



02 

 70 

 14 

 17 



6.05 



4.85 



o. 25 

 0.06 



1.44 



3 29 



4.80 

 o. 246 

 0.06 

 1.42 

 3.21 



20.62 20. oS 

 9.42 9.62 



Insoluble protein. — The insoluble protein was calculated from 

 nitrogen of the insoluble fraction (F3). The insoluble protein is 

 given in table II, and curves showing fluctuation during the growing 

 season are given in figs. 3-5. The insoluble protein falls and then 

 rises again at maturity in the root, while in the leaves the fluctua- 

 tion is in the opposite direction (see curves). In the stem the 

 decline is in the early stage from 10 . 89 per cent (June 3) to 6 . 03 per 

 cent (June 20). From that time on the curve is almost a straight 

 horizontal line; therefore the rate of synthesis of the insoluble 

 protein must have been keeping pace with the growth of the stem, 



