330 



BOTANICAL GAZETTE 



[NOVEMBER 



solution, in which i cc. represents 3 . i mg. of copper. The corre- 

 sponding equivalents of the different sugars expressed in milligrams 

 were found in the Munson-Walker table. The weight of sugar 

 found divided by the material used gives the amount of sugar con- 

 tained in I gm. of material. 



The soluble carbohydrates are in Fi and F^. The reducing 

 sugar was first determined. The non-reducing sugar was obtained 

 by subtracting the reducing sugar from the total sugar by hydro- 

 chloric acid hydrolysis at 67-69° C. for 10 minutes. 



The insoluble carbohydrates are in F3. They consist essentially 

 of colloidal polysaccharides, the greater part of which was starch. 

 The polysaccharides were determined by the FehHng solution after 

 acid hydrolysis for 2.5 hours with a reflex condenser. 



TABLE III 



The carbohydrates in the green plant 



Material 



Total carbohydrates . . . 

 Reducing carbohydrates 



Non-reducing 



Polysaccharides 



Total carbohydrates . . . 

 Reducing carbohydrates 



Non-reducing 



Polysaccharides 



Total carbohydrates . . . 

 Reducing carbohydrates 



Non-reducing 



Polysaccharides 



Roots 



Stems 



Branches 



Leaves 



June 3 collection (1-4 inches) 



10.00 10.10 



12 



76 12.57 



1.86 1.87 



2.14 2.05 



8.76 8.65 



June 20 collection (6-8 inches) 



6.78 6.61 



0.44 0.38 



0.63 0.67 



5-71 556 



July 8 collection (20 inches) 



10.51 10.41 

 Trace Trace 

 1.96 2.01 

 8.55 8.41 



The percentage of the diflferent carbohydrates of various organs 

 estimated at different times throughout the growth period is tabu- 

 lated in table III. Curves showing the changes in different sugar 

 content in these organs are given in figs. 6-8. These curves show 

 that in the roots the reducing sugars remain constant, while the 



