I9I9] 



KNUDSON &- SMITH— AMYLASE 



461 



The plants were grown under sterile conditions.^ The seeds 

 were sterilized by the calcium hypochlorite method (7) . After the 

 seeds were sterihzed, they were planted in test-tubes on sterilized 



^^.^-^^ 



Fig. I 



Fig. 2 



Figs, i, 2. — i, sterile culture tube; 2, germination tube; j, i per cent agar; 

 4, outer tube; 5, Erlenmeyer flask; 6, sterile cotton; 7, nutrient solution. 



agar, as shown in fig. i. When the seedKngs were sufficiently 

 developed, they were transferred to the culture vessels. The 

 culture vessels used were Erlenmeyer flasks of i or 2 1. capacity. 



= The writers are indebted to Dr. J. K. Wilson for the method of growing plants 

 under sterile conditions. 



