124 PHYSIOLOGY [Bot. Absts., Vol. V, 



891. Willamak, J. J. Colorimeter and indicator method. [Rev. of: Duggar, B. M., 

 and C. W. Dodge. The use of the colorimeter in the indicator method of H-ion determination 

 with biological fluids. Ann. Missouri Bot. Gard. 6: 61-70. 1919. (See Bot. Absts. 4, Entry 

 1449.)] Bot. Gaz. 68:232. 1919. 



892. Zellner, Julius. Zur Chemie der hoheren Pilze. XIII. Uber Scleroderma vul- 

 gare Fr. und Polysaccum crassipes DC. [Chemistry of the higher fungi.] Akad. Wiss. Wien 

 (Monatshefte fur Chemie) 39: 603-615. 1918. — Following the general plan of his earlier studies 

 the author reports the presence of mannit, cholin, and viscosin among the substances investi- 

 gated in Scleroderma. In Polysaccum it is noteworthy that no mannit occurs. With this 

 species special attention was devoted to a tannoid pigment. In neither fungus could the 

 author demonstrate either invertase, maltase, or diastase. — B. M. Duggar. 



S93. Zoller, H. F. Quantitative estimation of indole in biological media. Jour. Biol. 

 Chem. 41 : 25-36. 1920. — Indole is an important product of the metabolism of certain micro- 

 organisms. A simple, rapid, reliable method for its determination has been evolved, requir- 

 ing only the reagents and apparatus common to most laboratories. — G. B. Rigg. 



894. Zoller, H. F. Influence of hydrogen ion concentration upon the volatility of indole 

 from aqueous solution. Jour. Biol. Chem. 41: 37-44. 1920. — The range of most rapid vola- 

 tilization of indole from the aqueous solutions studied is from P H 8.0 to 10.5. Results suggest 

 that the practice of steam distillation can be supplanted by direct distillation when the reac- 

 tion of the solution is taken into account. — G. B. Rigg. 



METABOLISM (NITROGEN RELATIONS) 



S95. Anonymous. [Rev. of: Lakon, Georg. Der Eiweissgehalt panachierter Blatter, 

 gepriift mittels des makroskopischen Verfahrens von Molisch. (The protein content of mottled 

 leaves tested by the macroscopical method of Molisch.) Biochem. Zeitschr. 78: 145-154. 1917.] 

 Biedermann's Zentralbl. Agrikulturchem. 47: 251. 1918. — White-variegated leaves of many 

 species of plants, especially, Acer ncgundo, furnish suitable material for the macroscopical 

 demonstration of the protein reaction according to Molisch. The protein-rich green places 

 in the leaves give a very strong color while the protein-poor albescent places are only slightly 

 colored. Protein-rich and protein-poor places are directly related to the presence and absence 

 of chromatophores, in the leaf. In the case of yellow 'panachierten,' chromatophores are 

 found in the leaf tissues and so one finds them rich in protein. The investigation supports 

 the views of Molisch in that the principal masses of proteins of the leaves occur in the chro- 

 matophores. When submitted to the xanthoproteic reaction leaves which contain anthocya- 

 nin first take on a red color when placed in nitric acid solution, because, in spite of the decolor- 

 ization, they contain anthocyanin in the colorless isomeric form. — F. M. Schertz. 



896. Berman, N., and L. F. Rettger. Bacterial nutrition: further studies on the utili- 

 zation of protein and non-protein nitrogen. Jour. Bact. 3 : 367-388. 1918. — The utilization of 

 different brands of commercial peptones by proteolytic and non-proteolytic bacteria is prob- 

 ably related to the simpler nitrogen-containing substances. The liquefaction of gelatin was 

 not a necessary indication of the proteolytic property of an organism. The availability of 

 casein for bacterial use is shown before and after digestion with trypsin. — W. H. Chambers. 



897. Bonazzi, Augusto. On nitrification. III. The isolation and description of the 

 nitrite ferment. Bot. Gaz. 68: 194-207. pi. 14- 1919. — This paper presents the results of 

 the study of an organism, capable of forming nitrates from ammonia, isolated in a pure state 

 from Wooster [Ohio] soil after many unsuccessful attempts. A review is given of the per- 

 tinent literature, and the methods are described by which the organism was isolated and its 

 cultural characteristics determined. The cultural solution used throughout was the one rec- 

 ommended by Omelianski, of the following composition: H2O, 1000 cc. ; FeSO*, 0.4 gram; 

 MgSO«, 0.5 gram; K 2 HP0 4 , 1 gram; NaCl, 2 grams; and (NrL^SO*, 2 grams. Solid media 



