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mer of its life. A Northern-bred animal, that has ac- 

 quired immunity by having had an attack of Texas fever 

 within one year, may also be used as a source of blood 

 for inoculation. After securing the animal the follow- 

 ing instruments and articles should be prepared for the 

 inoculation : 



A sterilized hypodermic syringe, one or two sterilized 

 scalpels or sharp knives, one or two sterilized aspirat- 

 ino- needles with an inside diameter of 1 to 2 milli- 

 meters; a clean sterilized beaker or wide-mouth bottle, 

 containing a small glass rod, and the bottle or beaker 

 should be plugged with aseptic absorbent cotton; one 

 pair of scissors, a 2 per cent, solution of creolin, and 

 sterilized cotton or sponge, and sterilized distilled 

 water. The water may be sterilized by boiling one hour. 



Any or all of the above named articles, except the 

 creolin solution and water, may be sterilized by placing 

 them in a vessel of cold water, and then heating the 

 water until it boils for one hour. 



The animal from which the blood is to be drawn may 

 be secured by using a cattle nose-leader or by casting it 

 with ropes, hobbles, etc. Clip the hair very close over 

 a space 3 to 6 inches long and 2 inches wide along the 

 jugular forrow on either side of the neck (just over the 

 jugular vein). Wash the clipped skin with soap and 

 water; then with the creolin solution and then with dis- 

 tilled water. Now cord the neck of the animal as the 

 neck of a horse is corded just before it is to be bled. 

 When the neck is corded the jugular vein stands out 

 prominently. Now the aspirating needle, with its point 

 inclined toward the head, is pushed into the jugular vein 

 and the blood that escapes through the hollow needle is 

 caus^ht in the sterilized breaker or wide-mouth bottle, 

 and stirred slowly ^ith the glass rod, being careful to 



