252 



hold tbe <!otton plug over the mouth of the breaker or 

 bottle while stirring. As the fibrin collects in clots on 

 the jj^lass rod, it may be lifted out, and by a quick jerk 

 of the rod the clot is dislodged from the rod and the rod 

 is then returned to the breaker or bottle, and the blood 

 is stirred until no more fibrin collects on the glass rod. 

 In the breaker or bottle will remain nearly all of the red 

 blood cells floating in the blood serum and some of these 

 red blood cells will contain the micro-parasites that 

 cause Texas fever. This deferinated blood should be 

 kept warm ( above 90 degrees Fah. ) and when the sus- 

 <?eptible animals are ready for inoculation, the defebri- 

 nated blood may be drawn into the warm hypodermic 

 syringe and 1 cc injected under the skin of each sus- 

 ceptible animal. Remember that it is essential that the 

 defibrinated blood should be kept warm and that the 

 inoculations should be made as soon as possible after the 

 defirinated blood is prepared, because it may become 

 cool, or contaminated with septic or pus germs. It is 

 best to have the cattle that are to be inoculated con- 

 fined by halter or chains or stanchions in stalls. I 

 should not advise the use of defibrinated blood that is 

 over an hour old. 



In about six to ten days after the inoculation the tem- 

 perature of the inoculated animals will rise, ranging be- 

 tween 103 and 106 degrees Fah. The fever may con- 

 tinue from 3 to 15 or more days; then fall to normal 

 (102); a secondary fever usually begins about the 

 thirtieth day after the inoculation and may continue for 

 -several days. According to Pound, Francis and Conno- 

 way the primary inoculation fever appears in 6 to 10 

 ^ays, and the secondary inoculation fever appears about 

 the thirtieth day after the inoculation. The primary in- 

 oculation fever, as a rule, is more regular or will occur 

 with greater regularity than the secondary inoculation 



