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these cattle were too old to be inoculated with safety, 

 and the dose of defibrinated blood was too large for a 

 single or first inoculation. The strength of the blood of 

 an immune animal is never known until it is tested by 

 inoculation; hence it is always safest to use the mini- 

 mum dose in the beginning or the first time the blood is 

 used. All of these animals should have been collected at 

 one place so that there would have been no delay in the 

 inoculations following the drawing of the blood and the 

 defibrinating it. The vessels were not sufficiently steril- 

 ized. They should have been boiled at least for thirty 

 minutes, and for safety one hour. 



