igig] 



HAAS—RESPIRA TION 



353 



tion is nearly 2. Table II B shows the net electrical resistance. 

 It is seen that the material is dead before the end of 100 minutes. 



TABLE II 

 Control for II A: 9 periods (21 min. each) in sea water; solution 



RENEWED AT BEGINNING OF EACH PERIOD 



Period 



I 



2 



3 

 4 

 S 

 6 



7 

 8 



9 



Change in PH 



•37-7 

 •37-7 

 •37-7 

 ■37-7 

 •37-7 

 ■37-7 

 •37-7 

 •37-7 

 •37-7 



.67 = 0.70 

 .67 = 0.70 

 .70 = 0.67 

 .73 = 0.64 

 .73 = 0.64 

 .73 = 0.64 

 .72 = 0.65 



. 73 = 0-64 

 .72 = 0.65 



Total 



change 



inPH=A 



0.70 

 1 .40 

 2.07 

 2.71 



3-35 

 3-99 

 4.64 

 5-28 

 S-93 



pH-T3 , 



PQ 



2^3 .2 





o o. 



0.70 

 1 .40 



2. 10 



2.80 



3 50 

 4. 20 



4.90 

 5.60 

 6.30 





cr! 



I .0 



I .0 



0.99 



0,97 



0.96 



0.95 



0.9s 



0.94 



0.94 



Time 

 in min. 



21 

 42 

 63 

 84 

 los 

 126 



147 

 168 

 189 



Relative rate of 

 respiration 



0.70 

 0.70 

 0.67 

 0.64 

 0.64 

 0.64 

 0.65 

 0.64 

 0.65 



70=1.0 

 70=1 .0 

 70 = 0.95 

 70 = 0.91 

 70 = 0.91 

 70 = 0.91 

 70 = 0.93 

 70 = 0.91 

 70 = 0.93 



TABLE II A 

 Change in PH value of sea water produced by respiration of Laminaria during 2 



PERIODS (24 min. each) IN SEA WATER, AND DURING 7 SUBSEQUENT PERIODS IN 

 sea WATER CONTAINING 1 7. 4 PER CENT (bY VOLUMe) OF ACETONE 



Solution 



Sea water. 



Sea water 



17 -4 per 

 Sea water 



17.4 per 

 Sea water 



17.4 per 

 Sea water 



17.4 per 

 Sea water 



17.4 per 

 Sea water 



17.4 per 

 Sea water 



17.4 per 



contaming 

 cent acetone 

 containing 

 cent acetone 

 containing 

 cent acetone 

 containing 

 cent acetone 

 containing 

 cent acetone 

 containing 

 cent acetone 

 containing 

 cent acetone 



Period 



Change in PH 



8.37- 

 8.37- 



8.37- 



" 37- 



37- 



37- 



37- 



37- 



37- 



-7.76=0.61 

 -7.78 = 0.59 



-6.80 = 1.57 



-6.75 = 1.62 



-7.06 = 1.31 



-7.50=0.87 



-7.54 = 0.83 



-7.62=0.75 



-7.74=0.63 



3 



K o<: 



^ a II 



C fc- t) 

 — 3c 



S O 



=« J: rt 

 o 



1-57 

 319 

 4-5° 

 5-37 

 6.20 

 6-95 

 7.58 



r- P 



ffl 



MS 



6 



0.60 

 1 .20 



1.80 



2.40 

 3 00 

 3 -So 

 4.20 



§ II 



E o 



« 



2.61 

 2.66 

 2.50 

 2.24 

 2.07 

 1-93 

 1.80 



Time in 

 min. 



24 

 48 



72 



96 



120 



144 



168 



192 



216 



3-- 



E a 



H 



o 

 o 



24 



48 



72 



96 



120 



144 



168 



Relative rate of 

 respiration 



1-57-^0.60 = 2.6 

 1.62^0.60 = 2.7 



1 .31 -i-0.6o = 2.2 



0.87-^0.60 = 1.5 

 0.83^0.60 = 1.4 

 0.75-^0.60 = 1.3 

 0.63 -=-0.60 = 1.1 



The fact that respiration proceeds here at a rate much above the 

 normal (although death has taken place) is very clearly brought 



