No. 2, June, 1921] MORPHOLOGY AND TAXONOMY, FUNGI, ETC. 191 



quent intervals until a colony has developed from which tube cultures may be secured. — 

 When the object is simply to secure a pure culture from a single cell the following method is 

 used: Spread hot nutrient agar over sterilized glass slides; when cool, inoculate from a dilute 

 culture by means of a glass rod; cover the agar with a thin sheet of perforated celluloid; place 

 sterilized coverslips over the perforations and incubate; examine the circlets of medium in 

 the minute moist chambers until one is found which contains a single cell; center the colony 

 in the field, replace the lens with an accurately centered needle (method described), touch 

 the colony, and transfer in the usual way; examine to see that the colony has been touched. 

 [See also Bot. Absts. 8, Entry 1324.]— D. Reddick. 



1329. HoRT, Edward C. The reproduction of aerobic bacteria. Jour. Hygiene 18: 369- 

 408. PI. ^-7. 1920. — A culture oi Bacillus typhosus was secured from a single cell and studied 

 in 4 per cent glucose broth or 4 per cent glucose agar. Numerous types of organisms, which 

 are illustrated, developed in the cultures. This supports conclusions previously published 

 that the lower bacteria are able to reproduce themselves ' 'by the production of fertile branches 

 and buds, and by the endogenous production of gonidial bodies, in addition to the more 

 familiar method by equal binary fission." These are not regarded as involution forms. The 

 latter are defined as "strictly speaking, a sterile organism which is not only incapable of 

 maintaining its reproduction activity, but is also incapable of maintaining its integrity of 

 form."— Part 2 (p. 382 to 407) is entitled "the effect of the reproductive life of bacteria on the 

 agglutinability of bacterial emulsions." [See Bot. Absts. 8, Entry 1324.]— D. Reddick. 



1330. Kawakama, Koichiro, and Stjehiro Yoshida. Bacterial disease on Milletia 

 plant. (Bacillus milletiae n. sp.) Bot. Mag. Tokyo 34: 110-115. PL 2. 1920.— See Bot. 

 Absts. 7, Entry 1190. 



1331. KosER, Stewart A. A bacteriological study of canned ripe olives. Jour. Agric. 

 Res. 20: 375-379. 1921. — In a bacteriological examination of 480 commercial containers of 

 ripe olives, living microorganisms were found in practically every sample which showed 

 either a "swelled" condition or had a bad odor. Sixteen different kinds of organisms were 

 found. Bacteria were commonest, members of the colon group predominating. — Viable 

 organisms were found in a small percentage of containers which were ^'normal." "These 

 were either aerobic, spore-forming bacilli, cocci or apparently dormant members of the colon 

 group." — D. Reddick. 



1332. LoHNis, F., AND Roy Hansen. Nodule bacteria of leguminous plants. Jour. 

 Agric. Res. 20: 543-555. PI. 68-69. 1921. — The nodule bacteria of leguminous plants can be 

 divided into 2 groups. Each group is distinct, morphologically as well as physiologically. 

 The bacteria of the 1st group, which the authors consider to be Bacillus radicicola Beijerinck, 

 are peritrichic, grow with relative rapidity on agar plates, and produce very characteristic 

 changes in milk. They produce nodules on the roots of the following plants: Clover, sweet 

 clover, alfalfa, vetch, pea, navy bean, lupine, black locust, Amorpha, and Stropho styles. 

 The bacteria of the 2nd group are monotrichic, grow slowly on agar plates, and cause no 

 marked change in milk. They have been isolated from cowpea, soybean, peanut, beggar 

 weed, Acacia, Genista, and Cassia. The nomenclature of this latter group is discussed. 

 Bacillus radiobacter Beijerinck isolated from legume nodules was studied with the 2 groups 

 of nodule forming bacteria. — W. H. Burkholder. 



1333. Miller, H. M. Modification of the Howard method for counting yeasts, spores and 

 bacteria in tomato products. Jour. Indust. Eng. Chem. 12:766. 1920.— The modification 

 described is based on the fact that by boiling tomato pulp with LoefRer's methylene blue 

 and Tiehl-Neilsen's carbolfuchsine the microorganisms are stained a slightly deeper color 

 than the tomato tissues. — Henry Schmitz. 



1334. Truffatjt, G., et N. Bezssonoff. Sur les caracteres communs au Bacterium /S, 

 symbiote du Clostridium Pastorianum de Winogradsky, et au B. aliphaticum non liquefaciens 



