No. 2, Junk, 1921] PHYSK)LOGY 213 



283-290. 1920.— The following amino acids were found in the aqueous solution remaining 

 after the products of hydrolysis had been subjected to butyl alcohol extraction (Dakin's 

 method): Glutaminic acid, 19.07 per cent; aspartic acid, 5.12 per cent; alanine, 2.67 per cent; 

 serine 1.76 per cent. — G. B. Rigg. 



1473. Johns, C. O., and N. C. Waterman. Some proteins from the mung bean, Phaseolus 

 aureus Roxburgh. Jour. Biol. Chem. 44:303-317. 1920.— The mung bean contains about 

 21.74 per cent of protein. This yields 2 globulins distinguished from each other by marked 

 differences in their percentages of the basic amino acids. — G. B. Rigg. 



1474. Jones, D. B., and C. O. Johns. Hydrolysis of the globulin of the coconut, Cocos 

 nucifera. Jour. Biol. Chem. 44: 291-301. 1920.— The amino acids resulting from the hydroly- 

 sis were determined. Glytaminic acid was the most abundant. — G. B. Rigg. 



1475. Miller, E. R. Dihydroxyphenylalanine, a constituent of the velvet bean. Jour. 

 Biol. Chem. 44:481-486. 1920. 



1476. Paris, G. Studi e ricerche sulla biochimica del tobacco. III. Sul metabolismo 

 dell'azoto nello sviluppo della foglia di tabacco. [The biochemistry of tobacco. III. Nitrogen 

 metabolism in the growing leaf.] Staz. Sper. Agrarie Ital. 53: 81-96. 1920.— The direct con- 

 nection existing between nicotine production and nitrogen nutrition was demonstrated by 

 cutting tobacco leaves longitudinally in two equal parts and allowing one part to float on 

 distilled water, while the other was floated upon a 0.25 per cent solution of NH4CI in dif- 

 fuse light. The content of nicotine of the ammonium chloride portions was found in two cases 

 to be 2.22 and 2.01 respectively, while the natural nicotine content of the water portions was 

 1.88 and 1.54 per cent respectively. — The physiological effect of "topping" is demonstrated. 

 In the natural plant the total nitrogen and also the amide nitrogen (about 5 the total amount) 

 increased from the basal to the 5th whorl, while the nicotine content, averaging g the quan- 

 tity of total nitrogen, diminished. In the "topped" plant total nitrogen and nicotine con- 

 tent run about parallel and the latter is so increased in amount as to exceed total nitrogen, 

 and a low content of amide nitrogen is present. — As for soluble nitrogen, the observation was 

 made that, while in the "topped" plants the greater proportion was in the form of basic and 

 protein substances, in the natural plants the greater part of the soluble nitrogen was in the 

 amidic form. Therefore, "topping" of the plants leads to a modification of the chemical 

 composition of the leaves and the stem with an accumulation of nicotine and strong diminu- 

 tion of amides. According to the author, nicotine is a condensation product, the formation 

 of which is dependent upon the amides, light, and chlorophyll. — A. Bonazzi. 



METABOLISM (ENZYMES, FERMENTATION) 



1477. Arzberger, C. F., W. H. Peterson, and E. B. Fred. Certain factors that influence 

 acetone production by Bacillus acetoethylicum. Jour. Biol. Chem. 44:465-479. Fig. 1-2. 

 1920. — -This organism ferments glucose, sucrose, potato starch, and xylose in a peptone- 

 phosphate medium with the production of acetic, formic, and lactic acids, ethyl alcohol, 

 acetone, and carbon dioxide. — G. B. Rigg. 



1478. BotJRQUELOT, Em., et H. H^rissey. Presence dans le Melilot et I'Asperule odor- 

 ante, de glucosides fournissant de la coumarine sous I'action hydrolysante de I'emulsine. [The 

 presence in Melilotus and Asperula odorata of glucosides furnishing coumarine when hydrolyzed 

 with emulsin.] Compt. Rend. Acad. Sci. Paris 170: 1545-1550. 1920. — Coumarine is secured 

 by treating the glucosides of these plants with enzymes extracted from them. The same 

 result is obtained if emulsin is used, both sugar and coumarine being fonned from the glu- 

 cosides. — C. H. Farr. 



1479. Bradley, H. C, and H. Felsher. Studies of autolysis. VI. Effect of certain 

 colloids upon autolysis. Jour. Biol. Chem. 44: 553-561. 1920. 



