EXPERIMENT STATION REPORTS. 



155 



Immune pig No. 246, wt, 84 lbs., was hyperimmuuized with 7.5 cc. 3 

 hr. 25 cc. virus NaCl -f 5 cc. virus blood from virus pig No. 267, per lb. 

 of body wt. 



Autopsy of virus pig No. 267: Numerous petechiae on kidnej's. Liver 

 congested. Sjileen hemorrliagic. Lungs hemorrhagic. Inguinal glands 

 hemorrhagic and enlarged. Intestines congested. This jjig was killed 

 12 days after inoculation. 



Hyperimmune No. 246 was bled 3 times from the tail and then bled 

 to death from carotid arter}-, 2700 cc. of serum being secured and tested 

 as follows : 



Date. 



6-27-11 

 6-27-11 



6-27-11 



6-27-11 



11- 8-11 



11- 8-11 



11- 8-11 



Material injected. 



Result of test. 



2 cc. virus, No. 291 . 



10 cc. serum 



2 cc. virus, No. 291 . 



15 cc. 

 2 cc. 



20 cc. 

 2 cc. 



25 cc. 

 2 cc. 



30 cc. 

 2 cc. 



35 cc. 

 2 cc. 



serum 



virus, No. 291 



serum 



virus. No. 461 . 



serum 



virus. No. 461 . 



serum 



virus, No. 461 



serum 



virus, No. 461 . 



7-3-11, died. 



Autopsy: Good cholera lesions. 



7-3-11, killed in dying condition. 

 Autopsy: Lymph glands and lungs 

 rhagic. 



7-10-11, died. 

 Cholera lesions. 



7-10-11, killed. 



Good cholera lesions. 



11-2.5-11, died. 

 Cholera lesions. 



12-1-11, died. 

 Cholera lesions. 



Lived. 



hemor- 



(NoTE. — Three hour twenty-five cc. virus means virus injected into virus pig at rate of 25 cc. 

 per lb. of body wt. and let remain 3 hours, and so on. Immune pigs made hyperimmune have same 

 number.) 



Thus, according to our test of this serum it was not very potent. By 

 the virus i>ig showing good cholera lesions, I attributed this low potency 

 to the time the viiiis NaCl remained in abdomen of virus pig, 3 hrs. 

 not being long enough for the saline solution to gain enough virulence. 



Immune pig No. 247, wt. 90 lbs., was hyperimmunized with 5 cc. 3 

 hr. 25 cc. virus NaCl -|- 5 cc. virus blood, from virus pig No. 267, 

 per lb. of body wt. by the quick subcutaneous method. 



Autopsy of virus No. 267. See above. 



