156 



STATE BOARD OP AGRICULTURE. 



This hyperimmune was bled 3 times from the tail at interrals of 1 

 week and then bled to death, 2,900 cubic centimeters of serum being se- 

 cured. This senmi was tested as follows : 



Date. 



6-27-11 

 6-27-11 

 6-27-11 

 11- 8-11 

 11- 8-11 

 11- 8-11 

 11- 8-11 



Material injected. 



10 CO. serum 



2 cc. virus, No. 291 



15 cc. serum 



£ cc. virus, No. 291 



20 cc. serum 



2 cc. virus, No. 291 



25 cc. serum 



2 cc. virus, No. 461 



.'50 cc. serum 



2 cc. virus, No. 461 



35 cc. serum 



2 cc. virus. No. 461 



2 cc. virus, No. 461 



Result of test. 



7-6-11, died. 

 Cholera lesions. 



7-4-11, died. 

 Cholera lesions. 



7-10-11, died. 

 Cholera lesions. 



11-22-11, died. 

 Cholera lesions. 



11-23-11. 

 Cholera lesions. 



12-6-11, died. 

 Cholera lesions. 



11-27-11, killed. 

 Cholera lesions. 



Immune pig No. 250, wt. 90 lbs., M'as hyperimmunized with 10 cc. per 

 lb. of body wt. of 3 hr. 25 cc. virus NaCl No. 264 by quick subcutaneous 

 method. 



Autopsy of virus pig No. 264 : Few petechiae on cortex of kidneys. 

 Lymph glands enlarged and hemorrhagic. Spleen enlarged and of dark 

 color. Coecum ulcerated. Lungs hemorrhagic and intestines congested. 



Hyper No. 250 was bled 3 times from the tail and killed, bleedings being 

 made at intervals of one week. 3,000 cubic centimeters of serum were 

 secured and tested as follows: 



