EXPERIMENT STATION REPORTS. 



157 



The three preceding hyperimmunes are the only ones carried through 

 the process of serum manufacture with 3 hour virus NaOl. By autopsies 

 of virus pigs used showing good cholera lesions, I concluded that the 

 time the saline solution remained in abdominal cavities was too short 

 for the solution to gain enough virulence to prove successful in hyper- 

 immunizing, unless large quantities were used. 



4 HOUR 20 CC. VIRUS NaCl. 



Immune pig No. 329, wt. 65 lbs., was hyperimmunized by the quick 

 subcutaneous method with 10 cc. per lb. of body wt. of 4 hr. 20 cc. virus 

 NaCl from virus pig No. 433. 



Autopsy of virus pig No. 433 : Few petechiae on cortex of kidneys 

 and on auricles. Spleen enlarged, dark color, and hemorrhagic. In 

 guinal and sub-lumbal glands enlarged and hemorrhagic. Lungs hem- 

 orrhagic. Liver and intestines congested. 



Hyperimmune No. 329 was bled 4 times including neck bleeding at 

 intervals of one week, 2890 cc. of serum being secured which was tested 

 as follows: 



Check pig killed 9 days after inoculation. 



4 HOUR 25 CC. VIRUS NaCl. 



Pig No. 251, wt. 95 lbs., was hyperimmunized by the quick subcutane- 

 ous method with 7i/^ cubic centimeters of 4 hr. 25 cc, virus NaCl + 5 cc. 

 virus blood per lb. of body wt. from virus pig No. 272. 



Autopsy of virus pig No. 272: Kidneys enlarged and congested. 

 Coecum ulcerated. Lymph glands enlarged and congested. Liver of 

 dark color. Lungs hemorrhagic, and heart normal. 



Two thousand nine hundred cubic centimeters of serum were secured 

 from hyper No. 251 by bleeding 3 times from tail and then bleeding to 

 death from neck. This serum was tested as follows : 



