158 



STATE BOARD OF AGRICULTURE. 



Immune pig No. 252, Avt. 72 lbs., was liyperimmimized bv the quick 

 subcutaneous method with 5 cc. of 4 lir. 25 cc. vims NaCl -f- 5 cc. virus 

 blood per lb. of body wt. from virus pig No. 272. 



Autoi>sy of viinis pig No. 272 : See above. 



This hyjierimmune was bled 4 times including killing, 2200 cubic cen- 

 timeters of serum being secured and tested as follows: 



Immune pig No. 254, wt. 118 lbs., was hyperimmunized by quick sub- 

 cutaneous method with I2I/2CC. per lb. of body wt. of 4 hr. 25cc. virus 

 NaCl from virus pig No. 270. 



Autopsy of vims No. 270: Vvw petccliiae on kidaeys. Lymjih glands 

 enlarged. Spleen enlarged and hemorrhagic. Heart and lungs normal, 

 and liver congested. 



Hyper No. 254 was bled ?* times frcun tail, then killed. 3400 cc, seram 

 were secured and tested as follows: 



Immune pig No. 256, wt. lOfi lbs., was hyperimmunized by quick sub- 

 cutaneous method with 10 cc. 4 hr. 25 cc. virus NaCl No. 270. 



Autopsy of virus No. 270 : Numerous petechiae on kidneys. lug-uinal 

 and sub-lumbal glands hemorrhagic. Lungs hemorrhagic. Spleen en- 

 larged and hemorrhagic. Congested areas on intestines. 



The above hyperimmune was bled 3 times from tail, then killed, 

 cc. of serum were secured and tested as follows : 



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