EXPERIMENT STATION REPORTS. 



159 



Check pig died in 12 days from inoculation. 



Pig No. 257, wt. 120 lbs., was hyiierimmimized b3' quick subcutaneous 

 method with 5 cc. 4 hr. 25 cc. virus KaCl + 5 cc. virus blood per lb. from 

 virus pig No. 278. 



Autopsy of virus pig No. 278: Lymph glands enlarged and congested. 

 Liver congested. Spleen enlarged and congested. Kidneys containing 

 a few petechiae on cortex and medulla. Lungs hemorrhagic. 



Hyperimmune No. 257 was bled 8 times from tail and then killed. 

 4000 cc. of serum being secured which was tested as follows: 



Date. 



8-2-11 

 8-2-11 

 8-2-11 

 8-2-11 



Material injected. 



20 cc. serum 

 2 cc. virus. 



15 cc. serum 

 2 cc. virus. 



10 cc. serum 

 2 cc. vims. 



2 cc. virus. 



Result of test. 



Lived. 

 Lived. ' 



Lived. 



8-16-11. killed. 



Autopsy: Cholera lesions. 



Immune pig No. 258, wt. 82 lbs., Avas hyperimmunized with 5 cc. of 

 4 hr. 25 cc. -j- 5 cc. virus blood per lb. of body wt. from virus pig No. 

 278 by the quick subcutaneous method. 



Autopsy of virus pig No. 278: See above. 



This hyperimmune was bled 3 times from tail, then killed. 2700 cc. 

 of serum were secured and tested as follows: 



Immune x^ig No. 260, wt. 128 lbs., was hyperimmunized with 10 cc. per 

 lb. of body wt. of 4 hr. 25 cc. virus NaCl by the quick subcutaneous 

 method from virus pig No. 277. 



