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STATE BOARD OF AGRICULTURE. 



Autopsy of virus pig No. 277: Numerous petechiae on cortex and 

 medulla of kidneys. Inguinal and sublumbal glands hemorrhagic. Spleen 

 enlarged and hemorrhagic. Lungs hemorrhagic, and heart normal. 



Hyperimmune No, 260 was bled 4 times including killing, 4400 cc. 

 of serum being secured and tested as follows : 



Immune pig No. 330, wt. 80 lbs., was hyperimmunized with 10 cc. per 

 lb. of body wt. of 4 hr. 25 cc. virus NaOl from virus pig No. 461. 



Autopsy of virus pig No. 461 : Numerous petechiae on kidneys. In- 

 guinal and other glands enlarged and hemorrhagic. Spleen enlarged 

 with hemorrhagic areas. Lungs and heart normal. 



Hyperimmune No. 330 was bled 4 times including killing, 3000 cc. 

 of serum being secured and tested as follows : 



Check pig died 9 days after inoculation. Cholera lesions. 



We carried through the process of serum manufacture, as shown above, 

 one hyperimmune with 4 hr. 20 cc. virus NaCl used at rate of 10 cc. 

 per lb. of body wt. and 8 hyperimmunes with 4 hr. 2.5 cc. virus NaCl 

 used at rate of 10 cc. and 12yo cc. per lb. of body wt. all of which pro- 

 duced serum equal in potency to the Dorset-Niles serum, according to 

 our method of testing serum. 



4 HR. 30 cc. VIRUS NaCl. 



Immune pig No. 323, wt. 100 lbs., was hyperimmunized by the quick 

 subcutaneous method with 7 cc. of 4 hr. 30 cc. virus NaCl + 3 cc. of 

 virus blood per lb. of body wt. from virus pig No. 479. 



Autopsy of virus pig No. 479 : Kidneys were enlarged and congested. 

 Lymph glands enlarged and hemorrhagic. Spleen hemorrhagic. Liver 

 congested. Lungs hemorrhagic. 



