162 



STATE BOARD OF AGRICULTURE. 



Autopsies of all preceding hrperimmunes : All organs normal. Virus 

 well absorbed, leaving no abscesses. 



5 HR. 30 CC. VIRTS NaCl. 



Immune pig No. 269, wt. 10,5 lbs., was hyperimmuuized with. 5 hr. 30 

 CC. vinis NaCl as follows: 



5-21-'ll — Injected subcutaneously 1000 cc. virus NaCl from virus pig 

 No. 281. 



o-21-'ll — Injected subcutaneously olo cc. virus XaCl from virus pig 

 No. 283. thus making The total virus used 15 cc. per lb. of body wt. 



Autopsy of virus pig No. 281 : Few petechiae on kidneys. Liver con- 

 gested. Ulcers on intestines. Lymph glands and spleen enlarged. Heart 

 normal and lungs hemorrhagic. 



Autopsy of virus pig No. 283 : Numerous petechiae on kidneys. Lungs 

 hemorrhagic. Spleen enlarged and hemorrhagic. Intestines ulcerated. 



This hyperimmune was bled 3 times, then killed, 3800 cc, of senmi 

 being secured. Autopsy showed all organs to be normal and virus well 

 absorbed. This serum was tested as follows: 



Date. 



Pig Weight 

 No. I (Lbs.) 



Material injected. 



Restilt of test. 



S-1-11 I 672 I 



I 



8-1-11 ' 673 ! 



8-1-11 674 I 



72 I 20 cc. serum 



t 2 cc. virus I Lived. 



63 15 cc. serum. 

 2 cc. virus. . 



58i 



10 cc. serum . 

 2 cc. virus . . 



Lived. 



8-27-11, died. 



Autopsy: Cholera lesions. 



Check pig died 12 days after inoculation. Autopsy: Cholera lesions. 



Immune pig No. 268, wt. 135 lbs., was hyperimmunized by injecting 

 15 cc. per lb. of body wt. of 5 hr. 30 cc. virus NaCl as follows : 



5-22-'ll — Injected subcutaneouslv 1300 cc. virus from virus pig No. 

 281. 



5-29-'ll — Injected subcutaneously 725 cc. virus from virus pig No. 283. 



Autopsy of virus pigs No. 281 and No. 283 : See above. 



Hyperimmune No. 268 was bled 3 times from tail, then killed, 3150 cc. 

 of serum being secured and tested as follows: 



Check pig died 12 days after inoculation. Cholera lesions. 



Autopsy of hyper. No. 268 showed all organs normal and virus well 

 absorbed. 



