EXPERIMENT STATION REPORTS. 



163 



Pig No. 270, wt. 120 lbs., was hypcrimmunized by injecting 15 cc. per 

 lb. of body wt. of 5 hr. 30 cc. virus NaCl solution as follows: 



5-23-'ll — Injected subcutaneously 900 cc. virus NaCl from virus pigs 

 No. 281 and No. 280. 



5-29-'ll — Injected subcutaneously 900 cc. virus NaCl from virus pig 

 No. 283. See preceding autopsies of No's 281-283. 



Autopsy of viinis pig No. 280: Numerous petechiae on cortex and 

 medulla of kidneys. Lungs hemorrhagic. Liver congested. Spleen en- 

 larged, of dark color, and hemorrhagic. Lymph glands enlarged with 

 cortex hemorrhagic. 



Hyperimmune No. 270 was re-hyperimmunized, after being bled 3 

 times, by injecting subcutaneously 900 cc, 9 hr. 24 cc. virus NaCl from 

 virus pig No. 411. 



Autopsy of virus pig No. 411 : Spleen enlarged and congested. Kid- 

 neys enlarged and congested. Lungs hemorrhagic. Intestines congested. 



After re-hyperimmunizing, pig No. 270 was bled 3 times from tail and 

 then killed, making a total of 7 bleedings, and securing 4470 cc. of serum. 

 This hyper, aborted 2 pigs soon after the third bleeding and was given a 

 rest of 3 weeks before re-hyperimmunizing. Autopsy showed all organs 

 normal and virus well absorbed. Serum was tested as follows: 



Immune pig No. 262, wt. 160 lbs., was hyperimmunized as follows: 



5-20-'ll — Injected subcutaneouslv 1100 cc. 5 hr. 30 cc. virus NaCl No. 

 282. 



5-27-'ll — Injected subcutaneously 1300 cc. 5 hr. 30 cc. virus NaCl No. 

 285, making a total injection of 15 cc. per lb. of body wt. 



Autopsy of virus pig No. 282 : Numerous petechiae on kidneys. 

 Coecum ulcerated. Lymph glands enlarged and hemorrhagic. Spleen 

 enlarged and hemorrhagic. Lungs hemorrhagic. Liver and heart 

 normal. 



Autopsy of virus pig No. 285 : Numerous petechiae on kidneys. 

 Lymph glands enlarged and congested. Intestines ulcerated. 



Hyperimmune No. 262 was bled 4 times, 4500 cc. of serum being se- 

 cured. Autopsy of this pig showed all organs normal and virus well 

 absorbed. Serum was tested as follows : 



