164 



STATE BOARD OF AGRICULTURE. 



Immune pig No. 263, wi. 150 lbs., was hyperimmunized as follows: 



5-20-'ll — Injected subcnlaneoiisly 1100 cc. 5 lir. 30 cc. virus NaCl 

 from virus piu,- No. 282 (see above autopsy). 



5-27-"ll — Injected subcutaneouslv 800 cc. 5 hr. 30 cc. virus NaCl from 

 virus pig No. 285 (see preceding autopsy of No. 285), making a total 

 injection of 13 cc. per lb. 



This hyperimmune was bled 3 times from tail and then killed, 4G00 

 cc. of serum being secured. Autopt^y showed all organs normal. Serum 

 was tested as follows : 



Immune pig No. 290, wt. 120 lbs., was hyperimmunized by the quick 

 subcutaneous method by injecting 5 cc. of 5 hr. 30 cc. vims NaCl -|- 

 5 cc. of virus blood per lb. of body wt. from virus pig No. 292. 



Autoj^sy of virus pig No. 202 : Numerous petechiae on kidneys. Spleen 

 enlarged and congested. Inguinal and sublumbal glands enlarged and 

 congested. Lungs hemorrhagic. Liver badly congested. Heart normal. 

 Hyperimmune No. 290 was bled 4 times including killing, 3560 cc. of 

 serum being secured. Autopsy of this hyper, showed all organs normal. 

 Serum was tested as follows : 



Check pig was killed 11 days after inoculation. Autopsy: Cholera lesions. 



