EXPERIMENT STATION REPORTS. 



165 



Immune yi^ Xo. 291, wt. 120 lbs., was hTperimmunized by the quick 

 subcutaneous method by injecting 5 cc. 5 hr. :>0 cc. viru>s >«'aCl -f- 5 cc. 

 virus blood, per lb. of body wt. from virus pig No. 292. (See preceding 

 autojjsy of virus pig No. 292.) 



This hyperimmune was bled 3 times from arteries of tail, then killed, 

 3500 cc. of serum being secured and tested as follows: 



Check pig died 11 days after inoculation. Autopsy: Cholera lesions. 



Immune pig No. 271, wt. 120 lbs., was hyperimmunized as follows: 



5-23-'ll — Injected subcutaneously 850 cc. 5 hr. 30 cc. viinis NaCl from 

 virus pigs Nos. 280 and 281. (See above autojjsies of these virus pigs.) 



5-29-'ll — Injected 590 cc. 5 hr. 30 cc. vinis NaCl from virus pig No. 

 283, making a total injection of 12 cc. per lb. (See above autoi>sy of 

 virus pig No. 283.) 



This hyper, was bled 4 times, including killing, 3500 cc. of .sernm being 

 secured and tested as follows: r 



Date. 



Pig 

 No. 



Weight. 

 (Lbs.) 



Material injected. 



Result of test. 



8-29-11 .392 



8-29-11 



400 



8-29-11 421 



51 2 20 cc. serum 



2 cc. virus Lived. 



68 15 cc. serum 



I 2 cc. virus Lived. 



66 j 10 cc. serum | 



2 cc. virus i Lived. 



Check pig died within 12 days. 



Immune pig No. 322. wt. 90 lbs., was hyperimmunized by the quick 

 subcutaneous method by injecting 7 cc. of 5 hr. 30 cc. virus NaCl + 

 3 cc. of virus blood per lb. of body vrt. from virus pig No. 474. 



Autopsy of virus pig No. 474 : Petechiae on kidneys. Lymph glands 

 enlarged and hemorrhagic. Spleen hemorrhagic. Heart normal. In- 

 testines normal. Lungs hemorrhagic. 



Hyper No. 322 was bled 3 times from tail, then killed, 3000 cc. of 

 serum being secured. Autopsy of this hyper, showed all organs normal. 

 Slightly necrotic at points of virus injection. Serum was tested as 

 follows : 



