EXPERIMEXT STATION REPORTS. 



16: 



Check pig died within 10 days of inoculation: Cholera lesions. 



Immnne pig Xo. 334. wt 90 lbs., was hyperimmunized by the qiiuk 

 subcutaneous method by injecting 10 cc. per lb. of body wt. of o hr. -SO 

 cc. Tims ]S^aCl from virus pig Xo. 44:3. 



Autopsy of virus p>ig No. 443 : Numerous j>etechiae on kidneys. 

 Lymph glands enlarged and congested. >pleen enlai^ed and hemt rr- 

 hagic. Lungs hemorrhagic. 



Hyperimmune No. 334 was bled 4 times, including killing. 2670 cc. 

 of serum being secured and tested as follows: 



Date. PJg TtI^^^- 



No. : (Lite.) 



Material injected. 



Result of test. 



12-14-11 

 12-14-11 

 12-14-11 



409 



410 



411 



52 I 20 CC serum . 

 ! 2 cc Tims. . . 



53 i 15 cc serum . . 

 I 2 cc. Tims . - . 



LiTed. 



liTed. 



45 10 cc. semm j 12-2S-11. died. 



2 cc. Tims I Autopsy; Cholera lesions. 



Check pig died within 10 days from inoculation. Cholera lesions. 



5 HR. 45 CC. VIRUS NaQ. 



Immune pig Xo. 324. wt. 100 lbs., was hyperimmunized by the quick 

 subcutaneous method by injecting 10 cc. per lb. of body wt. of 5 hr. 

 45 cc. virus XaCl from virus pig Xo. 466. 



Autopsy of virus pig No. 466 : Few petechiae on kidneys. Lymph 

 glands enlai-ged with cortex hemorrhagic. Liver congested. Spleen en- 

 lai"ged and hemorrhagic. Heart normal. Lungs hemorrhagic. 



Hyperimmune No. 324 was bled 4 times, 27S5 cc. of serum being se- 

 cured. Autopsy of this animal showed virus to be well absorbed and 

 all orsrans normal. Serum was tested as follows: 



Date. ! Pig P^^^^^- 

 ' No. (Lbs.^ 



Material injected. 



12-4-11 



3SS 



12-4-11 



12-J-ll 3S4 



389 i 



104 

 97 

 S3 



20 cc. serum 

 2 cc. Tims. 



15 cc serum 

 2 cc Tims . 



10 cc serum 

 2 cc Tims . 



Result of test. 



LiTed. 



12-lS-ll. died. 



Autopsy: Cholera lesions. 



12-2S-11. died. 



Autopsy: Cholera lesions. 



Check pig died 9 days after inoculation: Autopsy: Cholera lesions. 



