EXPERIMENT STATION REPORTS. 



169 



Tissue slightly colored at points of injection. Serum secured was tested 

 as follows: 



6 HR 40 CC. VIRUS NaCL 



Immune pig No. 326, wt. 80 lbs., was hyperimmunized by the quick 

 subcutaneous method by injecting 10 cc. i)er lb. of body wt. of G hr. 

 40 cc. virus NaCl from virus pig No. 423. 



Autopsy of virus pig No. 423 : Few i>etechiae on kidneys. Lymph 

 glands enlarged and hemorrhagic. Heart and lungs normal. Intestines 

 congested. Spleen enlarged and containing hemorrhagic areas. 



Hyijerimmune No. 326 was bled 3 times from tail, then killed, 2895 cc. 

 of serum being secured. Autopsy of this hyper, showed all organs 

 normal. Serum was tested as follows: 



Immune pig No. 327 was hyperimmunized by the quick subcutaneous 

 method by injecting 10 cc. per lb. of body wt. of 6 hr. 40 cc. virus NaCl 

 from vinis pig No. 423. (See autopsy above.) 



This hyperimmune was bled 3 times from tail, then bled to death from 

 carotid arten,', 3195 cc. of serum being secured which was tested as fol- 

 lows : 



Check pig died within 10 days from inoculation. Cholera lesions. 



